PageRuler™ Prestained Protein Ladder是一个三色分子量标准,含有10种蛋白,分子量范围为10 – 170 kDa。该分子量标准含有两条参考条带,分别为70 kDa的橙色参考条带和10 kDa的绿色参考条带。
保存缓冲液
62.5 mM Tris-H3PO4 (pH 7.5, 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3和33%甘油。
质量控制
测试:Western杂交检测和SDS-聚丙烯酰胺凝胶电泳。
保存
4°C稳定保存3个月。如需保存更长时间,请-20°C保存。
图1。Migration patterns of PageRuler™ Prestained Protein Ladder in different electrophoretic conditions. The apparent molecular weight of each protein (kDa) has been determined by calibration of each protein against an unstained protein ladder in specific electrophoresis conditions. * Migration patterns were determined using respective NuPAGE® precast gels.