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Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glu

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上海金畔生物科技有限公司提供Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glu ，欢迎访问官网了解更多产品信息和订购。
Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glu

详情介绍

Gibco™ SILAC Advanced DMEM/F-12 Flex Media, no glucose, no phenol red

When supplemented with 1-2 % FBS, Advanced DMEM/F-12 Flex is capable of supporting cellular proliferation and maximum cell densities comparable to the conventional basal formulation supplemented with 5-10% FBS. Cells successfully cultured in Advanced DMEM/F-12 with no adaptation include Jurkat, WI-38, SP2, Vero, and MRC-5. We offer a variety of DMEM/F-12 modifications for a range of applications. Find the right formulation using the media selector tool.

This Advanced DMEM/F-12 is manufactured as follows:

With Without

• Non-essential amino acids; • Glucose;

• Sodium pyruvate; • Phenol red;

  • L-arginine;

  • L-glutamine;

  • L-lysine;

  • HEPES;

   


Advanced DMEM/F-12 is unique from other media due to addition of the following ingredients to allow for serum reduction: ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I lipid-rich bovine serum albumin for cell culture, and the trace elements sodium selenite, ammonium metavanadate, cupric sulfate, and manganous chloride. Advanced DMEM/F-12 typically requires supplementation with 1-5% Fetal Bovine Serum and 4 mM L-glutamine or GlutaMAX™ supplement (optional). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. The FBS concentration must be optimized for each cell line to obtain maximum serum reduction. Advanced DMEM/F-12 uses a sodium bicarbonate buffer system (3.7 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.


cGMP Manufacturing and Quality System:

SILAC Advanced DMEM/F-12 Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco™ SILAC DMEM Flex无糖，无酚红

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上海金畔生物科技有限公司提供Gibco™ SILAC DMEM Flex无糖，无酚红 ，欢迎访问官网了解更多产品信息和订购。
Gibco™ SILAC DMEM Flex无糖，无酚红

详情介绍

Gibco™ SILAC DMEM Flex无糖，无酚红

Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12. We offer a variety of Gibco™ DMEM modifications for a range of applications. Find the right modification using the media selector tool.


This DMEM is modified as follows:

With Without

 • Glucose

 • Phenol red

 • L-arginine

 • L-glutamine

 • L-lysine

 • Sodium pyruvate

 • HEPES


DMEM is unique from other media as it contains 4 times the concentrations of amino acids and vitamins than the original Eagle's Minimal Essential Medium. DMEM was originally formulated with low glucose (1 g/L) and sodium pyruvate, but is often used with higher glucose levels, with or without sodium pyruvate. SILAC DMEM Flex has been further modified to not contain lysine, arginine, and glutamine. DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. DMEM uses a sodium bicarbonate buffer system (3.7 g/L), so requires a 5-10% CO2 environment to maintain physiological pH.


cGMP Manufacturing and Quality System：

SILAC DMEM Flex is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no

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上海金畔生物科技有限公司提供Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no ，欢迎访问官网了解更多产品信息和订购。
Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no

详情介绍

Gibco™ SILAC RPMI 1640 Flex Media, no glucose, no phenol red

RPMI 1640 Medium is unique from other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 Medium contains biotin, vitamin B12, and PABA, which are not found in Eagle's Minimal Essential Medium or Dulbecco's Modified Eagle Medium. In addition, the vitamins inositol and choline are present in very high concentrations. RPMI 1640 Medium contains no proteins, lipids, or growth factors. Therefore, RPMI 1640 Medium requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). SILAC protein labeling experiments should be conducted using Gibco™ Dialyzed FBS. RPMI 1640 Medium uses a sodium bicarbonate buffer system (2.0 g/L), and therefore requires a 5-10% CO2 environment to maintain physiological pH.


cGMP Manufacturing and Quality System:

SILAC RPMI 1640 Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.