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Gibco™ ExpiCHO™ Stable Production AGT Medium

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上海金畔生物科技有限公司提供Gibco™ ExpiCHO™ Stable Production AGT Medium ，欢迎访问官网了解更多产品信息和订购。
Gibco™ ExpiCHO™ Stable Production AGT Medium

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Gibco™ ExpiCHO™ Stable Production AGT Medium

ExpiCHO SPM supports an easy transition from transient to stable production, provides high titers without the need for additional medium optimization (see figures below), and works well with existing EfficientFeed C+ supplement.


ExpiCHO SPM is part of the ExpiCHO family, an end-to-end solution that empowers you to carry out your entire workflow from transient to stable expression without having to screen or optimize media and feeds.


Hassle-free scale-up:

Simplify your process as you transition into stable clones with ExpiCHO SPM—no cell adaptation, no media optimization, no changes in cell doubling time and peak density. ExpiCHO SPM eases the transition using ExpiCHO-S cells and provides optimal performance with less need for fine-tuning from development scientists to get you to the next step faster. This medium supports high titers under standard fed-batch process conditions in conjunction with EfficientFeed C+ supplement. It is available in liquid format or in our AGT dry format (this page) from 10 L through 450 L equivalents with larger sizes available upon request.


ONE Solution:

The ExpiCHO family includes products that are engineered to work seamlessly with each other. ExpiCHO SPM is a member of this family and allows expanded support of bioproduction from transient to stable protein expression. Look for ExpiCHO-branded protocols and methods to get optimal performance in the development process.


Members of the ExpiCHO family include:

• ExpiCHO Stable Production Medium (this page)

• ExpiCHO Expression Medium

• ExpiCHO-S cells

• Expifectamine CHO Transfection Kit


The power to streamline transfer to manufacturing:

ExpiCHO SPM liquid format and AGT dry format demonstrate comparable cell growth and titer performance (see figure below). Therefore, scale-up and manufacturing using AGT dry format is simplified by knowing that the reconstituted AGT dry format will behave similarly to the liquid format used prior to scale-up. The AGT dry format is easy to reconstitute; just add water and it will automatically adjust pH and osmolality. This can help provide efficiencies in supply chain complexity, reconstitution complexity, and, therefore, overall expense.


When to use ExpiCHO Stable Production Medium:

• When you are using the ExpiCHO-S cell line for transient expression and want to scale up

• After you have completed the stable transfection of ExpiCHO-S cells in ExpiCHO Expression Medium. (Follow our recommended protocol.)

• When you do not have the time to perform medium adaptation or optimization


When NOT to use ExpiCHO Stable Production Medium:

• When you have an existing platform cell-line or feeds and are looking for a broad medium to support a wide variety of cell-lines

• When you need a medium for transfection. For transient and stable transfection, ExpiCHO Expression Medium is recommended.


Support available with ExpiCHO Stable Production Medium:

• A stable cloning protocol designed specifically for the ExpiCHO family of products (must be used for optimal performance)

• Licensing for the ExpiCHO-S cells for development of stable clones. 


Design your own media specific to your needs:

Whether it’s a bench-level experiment or large-scale bio-manufacturing process, we offer specialized formats, packaging, and quality levels specific to your needs. With Gibco BioProduction Services you can put the manufacturing power and years of technical experience of our R&D and Bioproduction Application Specialists to work for you. Reach out for consultation and/or services in medium, feed, or cell line development.

Gibco™ Diploid Production Serum-Free Medium (SFM)

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上海金畔生物科技有限公司提供Gibco™ Diploid Production Serum-Free Medium (SFM) ，欢迎访问官网了解更多产品信息和订购。
Gibco™ Diploid Production Serum-Free Medium (SFM)

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Gibco™ Diploid Production Serum-Free Medium (SFM)

Additionally, it supports growth of chicken embryo fibroblasts under serum-free conditions. Virus production can then be conducted under serum-free conditions without the addition of albumin or other supplements.


The Diploid SRM system is composed of two different kits:

1. Diploid Growth Serum-Reduced Medium (SRM) kit: A basal medium and 100X growth supplement designed for cell expansion

2. Diploid Production Serum-Free Medium (SFM) kit (this page): A basal medium and 100X production supplement designed for virus production


Human cells have traditionally been used to manufacture a variety of vaccines, such as the varicella zoster, polio, smallpox, hepatitis A, measles, mumps, and rubella vaccines. Traditionally, the only way to culture these cells has been to use classical media with 10% serum for growth and 1–5% human albumin (human sourced or recombinant) for virus production. These are two of the highest cost components in vaccine production. The Diploid SRM system helps reduce or eliminate both components while maintaining high virus titers.


Cost savings:

Reduction or elimination of serum can significantly reduce cost for a vaccine manufacturing process. Premium FBS is expensive with high price volatility. Given the typical cost pressures on vaccine production, both economic drivers make vaccine manufacturing to a target price extremely challenging. Diploid Growth SRM reduces the impact of this volatility in price and offers a level of cost consistency not available with processes that use serum in high volumes.


In addition, serum requires frozen storage. A 10% serum concentration in growth media can result in high frozen storage requirements for a vaccine process. A process requiring 1,000 L of growth medium would require the frozen storage of 100 L of serum. The Diploid Growth SRM kit comes with a 100X frozen supplement, enabling an 80% reduction in frozen storage requirements. 1,000 L of Diploid Growth SRM would only need 20 L of frozen storage: 10 L for FBS and 10 L for the 100X supplement.


Albumin is commonly added to virus production media. With the Diploid Production SFM, viruses can be produced at comparable titers without any additional supplementation.

Gibco™ LV-MAX™ Production Medium

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上海金畔生物科技有限公司提供Gibco™ LV-MAX™ Production Medium ，欢迎访问官网了解更多产品信息和订购。
Gibco™ LV-MAX™ Production Medium

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Gibco™ LV-MAX™ Production Medium

• Supports growth of suspension Viral Production Cells to densities of over 1.0 x 107 cells/mL

• Enables sustained, high-level production of high-density transiently transfected cultures, achieving titer of up to 1 x 108 TU/mL (unconcentrated LVVs-GFP)

• Supports growth and transfection of Viral Production Cells in culture formats from 96-deep well blocks to 2-L flasks and bioreactors


LV-MAC Production Medium is ready to use, with no supplementation required. The chemically defined formulation results in high reproducibility and lot-to-lot reliability. The medium is not recommended for adherent cell cultures.