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NEBExpress Ni-NTA 磁珠 货 号 #S1423L NEB酶试剂 New England Biolabs

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产品资料 – 蛋白表达和纯化技术 – 表达系统 ：磁性基质和磁分离架

NEBExpress Ni-NTA 磁珠                              收藏

货 号
规 格
价 格(元)
北京库存
上海库存
广州库存
成都库存
苏州库存

#S1423L
5 ml
10,899.00
无
无
无
无
无

#S1423S
1 ml
2,299.00
有
无
有
无
无

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 NEBExpress Ni-NTA 磁珠 货 号 #S1423L

相关产品

抗 MBP 磁珠

 

产品描述

几丁质磁珠：一种亲和介质，能少量分离纯化与 CBD（Chitin Binding Domain）融合的目的蛋白。由于该磁珠有一个磁力核心，因此可以从细胞培养上清液中磁力分离与 CBD 融合的蛋白。再生之后磁珠不会影响结合能力。随后，被磁珠结合的融合蛋白可以用于从细胞裂解液中捕获与之发生相互作用的靶蛋白。

 
几丁质磁珠以 50:50(v/v)的浓度悬浮贮存于含 20% 乙醇的水溶液中。
 
Amylose 磁珠：一种亲和介质，能少量分离纯化与 MBP（麦芽糖结合蛋白）相融合的目的蛋白。本品通过将 Amylose 共价耦联到一种顺磁颗粒上而形成，由于该共价结合可以在很宽的 pH 范围内保持稳定，使得 Amylose 磁珠可以从细胞培养上清液中分离 MBP 融合蛋白。随后，被磁珠结合的融合蛋白可以用于从细胞裂解液中捕获与之发生相互作用的靶蛋白。
 
Amylose 磁珠以 25 mg/ml 的浓度悬浮贮存于含 20% 乙醇的水溶液中。
 
Ni-NTA磁珠：一种亲和介质，能够少量分离纯化带有多组氨酸标签（His-tagged）的融合蛋白，可应用于手动操作或磁蛋白自动纯化模式。使用 Ni-NTA 磁珠用于固定化金属亲和色谱法（IMAC）纯化蛋白时，无论是未变性或变性条件下，均可有效结合并纯化不溶性蛋白质、聚集在包涵体中的蛋白质、或具有隔离聚组氨酸亲和标记的三级结构的蛋白。随后，被磁珠结合的 His-tagged 蛋白质可用于 pull-down 实验中捕获与其相互作用的蛋白质。
 
支持介质：几丁质磁珠是直径约为 10-100 μm 的超顺磁微粒。
 
Amylose 磁珠是直径约为 1-10 μm 的超顺磁微粒。
 
Ni-NTA 磁珠是琼脂糖基超顺磁球形微粒，大小约为20-100 μm。
 
结合容量：1 ml 几丁质磁珠能结合 2 mg CBD 融合蛋白。
 
1 mg Amylose 磁珠能结合 10μg MBP 融合蛋白。
 
Ni-NTA：因纯化目标不同而异，通常 1ml 柱床体积结合≥7.5 mg His-tagged融合蛋白。

mPEG NTA Cat. No. PG1-NTA-5k 5000 Da 50 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG NTA

Cat. No. PG1-NTA-5k
Specification 5000 Da
Unit Size 50 mg
Price $325.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

mPEG NTA Cat. No. PG1-NTA-2k 2000 Da 50 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

mPEG NTA

Cat. No. PG1-NTA-2k
Specification 2000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

NTA PEG Biotin Cat. No. PG2-BNNT-5k 5000 Da 50 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

NTA PEG Biotin

Cat. No. PG2-BNNT-5k
Specification 5000 Da
Unit Size 50 mg
Price $325.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

NTA PEG FITC Cat. No. PG2-FCNT-5k 5000 Da 5 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

NTA PEG FITC

Cat. No. PG2-FCNT-5k
Specification 5000 Da
Unit Size 5 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

4A arm PEG NTA Cat. No. PG4A-NTA-20k 20000 Da 50 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

4A arm PEG NTA

Cat. No. PG4A-NTA-20k
Specification 20000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

NTA PEG Biotin Cat. No. PG2-BNNT-3k 3400 Da 50 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

NTA PEG Biotin

Cat. No. PG2-BNNT-3k
Specification 3400 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

NTA PEG Biotin Cat. No. PG2-BNNT-10k 10000 Da 50 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

NTA PEG Biotin

Cat. No. PG2-BNNT-10k
Specification 10000 Da
Unit Size 50 mg
Price $385.00

Qty Add to Cart

Description:

Nanocs’ metal chelate PEG conjugates were made to chelate metal ions for biomedical applications. Pegylated metal chelators have strong afinity with many metal ions and they are water soluble. Conjugates were provided in lyophilized powder form.

Physical Properties:

  • Lyophilized powder form;
  • Soluble in regular aqeous solution and common organic solvents;

Storage Conditions:

  • Store at -20 0C.
Documents
  • SDS
  • DataSheet

NTA PEG Maleimide Cat. No. PG2-MLNT-5k 5000 Da 25 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

NTA PEG Maleimide

Cat. No. PG2-MLNT-5k
Specification 5000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

NTA PEG Maleimide is one of Nanocs’ thiol reactive PEG derivatives that can bind to metal ions and react with free thiol groups. NTA (nitrilotriacetic acid) is a commonly used and well characterized metal chelator. Ni-NTA resin has been used widely for his-tag protein purification. Maleimide functionalized NTA PEG can react readily with free thiol groups at pH 6.5~7.5 to form a stable thioether bond. Reaction between maleimide and thiol groups allows easy and quick conjugation of NTA tags to resins, particles and other material surfaces. PEG linker between NTA and maleimide group offers better water solubility, less steric hindrance and enhanced stability.

Product Specifications:

  • Composition: NTA PEG Maleimide
  • Appearance: Colorless solid;
  • Solubility: Soluble in aqueous buffer;
  • Reactive group: Maleimide;
  • Reactive toward: Sulfhydryl (-SH);

Storage Conditions:

  • Store at -20 0C. Desiccate.
Documents
  • SDS
  • DataSheet

Maleimide PEG NTA Cat. No. PG2-MLNT-3k 3400 Da 25 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Maleimide PEG NTA

Cat. No. PG2-MLNT-3k
Specification 3400 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

NTA PEG Maleimide is one of Nanocs’ thiol reactive PEG derivatives that can bind to metal ions and react with free thiol groups. NTA (nitrilotriacetic acid) is a commonly used and well characterized metal chelator. Ni-NTA resin has been used widely for his-tag protein purification. Maleimide functionalized NTA PEG can react readily with free thiol groups at pH 6.5~7.5 to form a stable thioether bond. Reaction between maleimide and thiol groups allows easy and quick conjugation of NTA tags to resins, particles and other material surfaces. PEG linker between NTA and maleimide group offers better water solubility, less steric hindrance and enhanced stability.

Product Specifications:

  • Composition: NTA PEG Maleimide
  • Appearance: Colorless solid;
  • Solubility: Soluble in aqueous buffer;
  • Reactive group: Maleimide;
  • Reactive toward: Sulfhydryl (-SH);

Storage Conditions:

  • Store at -20 0C. Desiccate.
Documents
  • SDS
  • DataSheet

NTA PEG Maleimide Cat. No. PG2-MLNT-2k 2000 Da 25 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

NTA PEG Maleimide

Cat. No. PG2-MLNT-2k
Specification 2000 Da
Unit Size 25 mg
Price $385.00

Qty Add to Cart

Description:

NTA PEG Maleimide is one of Nanocs’ thiol reactive PEG derivatives that can bind to metal ions and react with free thiol groups. NTA (nitrilotriacetic acid) is a commonly used and well characterized metal chelator. Ni-NTA resin has been used widely for his-tag protein purification. Maleimide functionalized NTA PEG can react readily with free thiol groups at pH 6.5~7.5 to form a stable thioether bond. Reaction between maleimide and thiol groups allows easy and quick conjugation of NTA tags to resins, particles and other material surfaces. PEG linker between NTA and maleimide group offers better water solubility, less steric hindrance and enhanced stability.

Product Specifications:

  • Composition: NTA PEG Maleimide
  • Appearance: Colorless solid;
  • Solubility: Soluble in aqueous buffer;
  • Reactive group: Maleimide;
  • Reactive toward: Sulfhydryl (-SH);

Storage Conditions:

  • Store at -20 0C. Desiccate.
Documents
  • SDS
  • DataSheet