McCoy’s 5A培养基 GlutaMAX™补充剂

上海金畔生物科技有限公司提供McCoy’s 5A培养基 GlutaMAX™补充剂 ,欢迎访问官网了解更多产品信息和订购。
McCoy’s 5A培养基 GlutaMAX™补充剂

详情介绍

McCoy's 5A培养基 GlutaMAX™补充剂

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator.


cGMP Manufacturing and Quality System:

Gibco™ McCoy's 5A is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


Gibco™ McCoy's 5A (modified) requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). Gibco™ McCoy's 5A (modified) uses a sodium bicarbonate buffer system (2.2 g/L) and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Gibco™ McCoy’s 5A (Modified) Medium

上海金畔生物科技有限公司提供Gibco™ McCoy’s 5A (Modified) Medium ,欢迎访问官网了解更多产品信息和订购。
Gibco™ McCoy’s 5A (Modified) Medium

详情介绍

Gibco™ McCoy's 5A (Modified) Medium

Using McCoy's 5A (modified) Medium:

Dr. Thomas McCoy originally formulated McCoy's 5A medium as a modification of Basal Medium 5A. Unlike other media, McCoy's 5A contains the reducing agent glutathione, bacto-peptone, and a high level of glucose. This product also includes Dr. Hsu's addition of Hanks' salts to enable use outside a CO2 incubator. McCoy's 5A (modified) Medium requires serum supplementation, commonly with 10% Fetal Bovine Serum (FBS). McCoy's 5A (modified) Medium uses a sodium bicarbonate buffer system (2.2 g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.


cGMP manufacturing and quality system:

McCoy's 5A (modified) Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards. For supply chain continuity, we offer an identical McCoy's 5A (modified) Medium product made in our Scotland facility (26600-080). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard.


McCoy′s 5A Medium

上海金畔生物科技有限公司提供McCoy′s 5A Medium ,欢迎访问官网了解更多产品信息和订购。
McCoy′s 5A Medium

详情介绍

McCoy′s 5A Medium

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:NaHCO3: 2.2 g/L;phenol red: 0.011 g/L;L-glutamine: no;glucose: 3.0 g/L;glucose: 3 g/L.

Application:Used for the primary culture of cells from a wide range of tissues. Recommend for production of virus in primary cell cultures.

Biochem/physiol Actions:McCoy′s 5A Medium has been originally developed to support liver tumor cells by modification of the amino acids found in BME. This formulation has also been used to support the growth of primary cultures of bone marrow, skin, gingiva, kidney, omentum, adrenals, lung, spleen, rat embryos, and other cell types.

McCoy′s 5A Medium

上海金畔生物科技有限公司提供McCoy′s 5A Medium ,欢迎访问官网了解更多产品信息和订购。
McCoy′s 5A Medium

详情介绍

McCoy′s 5A Medium

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:L-glutamine: 0.21 g/L;phenol red: 0.011 g/L;NaHCO3: 2.2 g/L;glucose: 3.0 g/L (Dextro).

General description:The amino acid requirements for in vitro c*tion of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Application:McCoy′s 5A Medium has been used:

to culture HCT-116?cells

to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1

to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

McCoy′s 5A Medium

上海金畔生物科技有限公司提供McCoy′s 5A Medium ,欢迎访问官网了解更多产品信息和订购。
McCoy′s 5A Medium

详情介绍

McCoy′s 5A Medium

sterility:sterile-filtered.

form:liquid.

technique(s):cell culture | mammalian: suitable.

impurities:endotoxin, tested.

components:L-glutamine: 0.21 g/L;phenol red: 0.011 g/L;NaHCO3: 2.2 g/L;glucose: 3.0 g/L (Dextro).

General description:The amino acid requirements for in vitro c*tion of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

Application:McCoy′s 5A Medium has been used:

to culture HCT-116?cells

to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1

to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies