标签归档：lectin

Soybean lectin (Glycine Max), FITC labeled Cat. No. SB1-FC-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), FITC labeled

Cat. No. SB1-FC-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Soybean lectin (Glycine Max), Rhodamine labeled Cat. No. SB1-RB-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), Rhodamine labeled

Cat. No. SB1-RB-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Soybean lectin (Glycine Max), Cy3 labeled Cat. No. SB1-S3-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), Cy3 labeled

Cat. No. SB1-S3-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

Soybean lectin (Glycine Max), Cy5 labeled Cat. No. SB1-S5-1 1 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Soybean lectin (Glycine Max), Cy5 labeled

Cat. No. SB1-S5-1
Specification
Unit Size 1 mg
Price $385.00

Qty Add to Cart

Description:

Soybean lectin (Glycine Max) is one of lectins that specifically binds tobeta N-acetyl glucosamine, galactose and mannose sugar residues. Nanocs provides a variety of chemically functionalized and bioconjugated Soybean lectin (Glycine Max) with different functionality. These functional wheat germ lectin products were purified by size chromatography to remove non-conjugated molecules and ensure adequate applications both in-vitro and in-vivo.

Specifications:

  • 2~7 functional groups on each Soybean lectin (Glycine Max) molecule.
  • Soybean lectin and conjugates provided either in water solution and dried powder;

Storage Conditions:

  • Store at 4 0C. Protect from light.

扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

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扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

        凝集素（Lectin）是指一种从各种植物，无脊椎动物和高等动物中提纯的糖蛋白或结合糖的蛋白，因其能凝集红血球（含血型物质），故名凝集素。常用的为植物凝集素（Phytoagglutin，PNA），通常以其被提取的植物命名，如刀豆素A（Conconvalina，ConA）、麦胚素（Wheat germ agglutinin，WGA）、花生凝集素（Pehaiut agglutinin，PNA）和大豆凝集素（Soybehai agglutinin，SBA）等，凝集素是它们的总称。

扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

通过盐析法、离子交换层析、凝胶过滤、亲和层析一系列操作对提取物进行分级分离，筛选富集对H5N1具有最强微中和活性的组分，通过电泳发现为包含五种肽段的寡聚蛋白，质谱分析显示该蛋白质为扁豆凝集素（FRIL）。已有研究表明扁豆凝集素单体具有一个糖结合结构域，但在溶液中的状态未得到确定，综合利用多角度光散射、动态光散射、电镜复染，发现扁豆凝集素为四聚体结构。

扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

进一步在体外定量测定了扁豆凝集素的抗病毒活性，空板减少试验与微中和试验显示PRNT50与EC50约0.7ug/Ml。利用11种流感毒株测定中和谱，发现除实验室菌株A，扁豆凝集素能以纳摩尔水平抑制不同亚型毒株。研究者通过鼻腔给药在小鼠体内评估凝集素的作用，凝集素抗病毒活性表现剂量依赖，高剂量给药能够明显延长存活时间甚至保护免受感染。

扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

扁豆凝集素抗病毒活性

扁豆凝集素是已知的甘露糖/葡萄糖特异性凝集素，但关于扁豆凝集素与复杂糖基化的结合机制还有待了解。研究者将接种流感病毒的鸡胚进行α–甘露糖酶抑制剂并在回收时利用切割高甘露糖的内切糖苷酶处理，纯化后利用串联质谱、免疫印迹分析。

 结果发现扁豆凝集素主要结合流感病毒血凝素，而且主要结合复合型糖链而不是高甘露糖链，这与其他凝集素有很大不同。为了探明这现象背后的机制，研究者利用荧光探针标记的扁豆凝集素，发现与1,3或1,4岩藻糖基化N乙酰葡萄胺的复合型糖链结合较好。

扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

凝集素结合糖链

进一步分析了扁豆凝集素的抗病毒机制,竞争性微中和试验发现甲基甘露糖苷和葡萄糖能够抑制其中和作用，证实了抗病毒中和效应依赖于糖结合功能。细胞用扁豆凝集素、流感病毒依次分开处理，不能有效中和病毒感染，说明扁豆凝集素抑制病毒进入，必须直接结合病毒粒子才能发挥作用。流感病毒侵入细胞主要是吸附、内吞、脱壳、核转运这几个步骤，通过血凝抑制试验发现扁豆凝集素不能抑制病毒的吸附，研究者通过低温减慢病毒内吞入胞，使病毒吸附在细胞上，加入扁豆凝集素后观察到明显的中和效果，而在常温下病毒发生内吞后无法发挥作用，因此凝集素作用于吸附与内吞之间的过程。利用病毒核蛋白抗体免疫荧光示踪病毒入胞过程，发现凝集素处理组导致病毒滞留在核周区，通过内体/溶酶体染色显示病毒停留在晚期溶酶体内，凝集素在溶酶体仍能保持活性，可能发挥阻止病毒核转运的作用。进一步研究者发现核蛋白免疫荧光表现为大颗粒，且随着凝集素浓度增加颗粒逐渐变大，推测是凝集素与病毒颗粒间形成多价交联，聚集成团，从而发挥抗病毒作用。

扁豆凝集素填料LCA/LcH Lens culinaris lectin的应用

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