标签归档：glucuronidase

a-D-葡萄糖醛酸酶检测试剂盒 α-Glucuronidase Assay Kit 货号：K-AGLUA Megazyme试剂盒

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a-D-葡萄糖醛酸酶检测试剂盒

英文名：α-Glucuronidase Assay Kit

货号：K-AGLUA

规格：50 Assays per kit

市场价： 3800元

The Alpha-D-Glucuronidase test kit is a simple, reliable and accurate method for the measurement and analysis of alpha-D-glucuronidase in various enzyme preparations. Containing and aldouronic acid substrate and an α-D-glucuronidase control.
Suitable for manual and microplate formats.

UV-method for the measurement of α-D-Glucuronidase in
various enzyme preparations

Principle:
(α-D-glucuronidase)
(1) Aldouronic acid (tri:tetra:penta) + H2O →
β-(1,4)-D-xylo-oligosaccharides + D-glucuronic acid

(uronate dehydrogenase; UDH)
(2) D-Glucuronic acid + NAD+ + H2O → D-glucarate + NADH + H+

Kit size:                            50 assays (manual) / 200 (microplate)
Method:                            Spectrophotometric at 340 nm
Reaction time:                  ~ 25 min
Detection limit:                 17 mU/mL
Application examples: 
Enzyme preparations and other materials
Method recognition:          Novel method

Advantages

  • Very competitive price (cost per test)
  • All reagents stable for > 2 years as supplied
  • Simple format
  • Mega-Calc™ software tool is available from our website for hassle-free raw data processing
  • Standard included
  • Suitable for manual, microplate and auto-analyser formats

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Beta葡萄糖醛酸酶[大肠杆菌] Beta-Glucuronidase (E.coli) 货号：E-BGLAEC Megazyme试剂盒

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Beta葡萄糖醛酸酶[大肠杆菌]

英文名：Beta-Glucuronidase (E.coli)

货号：E-BGLAEC

规格：500000 Units

市场价： 3816元

High purity recombinant beta-Glucuronidase (Escherichia coli) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.31
CAZy Family: GH2

Recombinant. From Escherichia coli. Supplied in solution (Tris.HCl / NaCl / EDTA).
Hydrolysis of non-reducing terminal β-D-glucuronic acid residues from glycoproteins and oligosaccharides of glycoconjugates.

Specific activity: ~ 35,000 U/mg (37oC, pH 6.8 on phenolphthalein-β-D-glucuronide); 
                           ~ 100 U/mg (37oC, pH 7.5 on pNP-β-D-glucuronide).

Store at 4oC. 
Do Not Freeze/Thaw 

Custom quantities and formulations available up on request. 

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Alpha葡萄糖醛酸酶[分离嗜热菌] α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant) 货号：E-AGUBS Megazyme试剂盒

发表于由
Alpha葡萄糖醛酸酶[分离嗜热菌]

英文名：α-Glucuronidase (Geobacillus stearothermophilus) (Recombinant)

货号：E-AGUBS

规格：200 Units at 70°C

市场价： 2600元

High purity recombinant alpha-D-Glucuronidase (Geobacillus stearothermophilus) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

EC 3.2.1.139
CAZy Family: GH67

Recombinant from Geobacillus stearothermophilus. 
In 3.2 M ammonium sulphate.
Hydrolysis of the α-1,2 glycosidic bond between D-glucuronic acid or it’s ether 4-O-methyl-D-glucuronic acid and 
D-xylose residues of xylo-oligosaccharides (aldouronic acids) from xylan.

Specific activity: ~ 7 U/mg (70oC, pH 7.0 on aldouronic acid).

Stability > 2 years 4oC.

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