标签归档：aldehyde

Biotin PEG aldehyde Cat. No. PG2-ALBN-5k 5000 Da 10 mg修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Biotin PEG aldehyde

Cat. No. PG2-ALBN-5k
Specification 5000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

  • Appearance: White/off-whilte solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reacive group: Aldehyde (-CHO);
  • Reactive to: N-terminal amine, primary amine, etc.;
  • Functional moiety: D-biotin;
  • Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

  • Store at -20 0C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Biotin PEG aldehyde, Aldehyde PEG Biotin Cat. No. PG2-ALBN-3k 3400 Da 10 mg修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Biotin PEG aldehyde, Aldehyde PEG Biotin

Cat. No. PG2-ALBN-3k
Specification 3400 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

  • Appearance: White/off-whilte solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reacive group: Aldehyde (-CHO);
  • Reactive to: N-terminal amine, primary amine, etc.;
  • Functional moiety: D-biotin;
  • Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

  • Store at -20 0C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Biotin PEG aldehyde, Aldehyde PEG Biotin Cat. No. PG2-ALBN-2k 2000 Da 10 mg修饰性聚乙二醇

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Biotin PEG aldehyde, Aldehyde PEG Biotin

Cat. No. PG2-ALBN-2k
Specification 2000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Biotin PEG Aldehyde

Biotin PEG Aldehde (Biotin-PEG-CHO) is one of Nanocs’ chemical reactive biotin PEG derivatives that can label N-terminal amines. Aldehyde group is reactive toward amine, hydrazide or oxyamine groups from pH 5-9. Unlike amine reactive succinimidyl ester group (NHS), aldehyde can react with protonated amines at acidic environment (pH ~4), a condition sometimes required for certain site-specific biotinylation reactions. Aldehyde reacts with amine group to form an intermediate Schiff bond. Further reduction with hydride will form a stable C-N bond. Reaction between aldhyde and other groups allows site-specific conjugation and labeling of biotin tag to desired position on targeted molecules. Resulted biotin can recognize and bind to avidin, streptavidin or neutravidin with high affinity. PEG linker between Biotin and aldehyde offers higher water solubility, less steric hindrance and enhanced stability. Compared to other commonly used biotinylation reagents, Nanocs pegylated biotins are water soluble, more stable and easier to use.

Physical Properties:

  • Appearance: White/off-whilte solid;
  • Solubility: Soluble in water, DMSO, DMF;
  • Reacive group: Aldehyde (-CHO);
  • Reactive to: N-terminal amine, primary amine, etc.;
  • Functional moiety: D-biotin;
  • Reactive toward: avidin, streptavidin or neutravidin;

Storage Conditions:

  • Store at -20 0C. Desiccate.

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Biotin PEG aldehyde over the amount of amine-containing molecules (such as protein or antibody) results in sufficient conjugation.

Materials Required:

  • Reaction buffer: Amine-free aqueous buffer, pH 5.0~6.5.
  • Biotin PEG aldehyde stock solution: 5-10 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve ready labeling molecules in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add Biotin PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Maleimide PEG aldehyde Cat. No. PG2-ALML-5k 5000 Da 10 mg修饰性聚乙二醇

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Maleimide PEG aldehyde

Cat. No. PG2-ALML-5k
Specification 5000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • White/off-white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
  • Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Maleimide PEG aldehyde Cat. No. PG2-ALML-3k 3400 Da 10 mg修饰性聚乙二醇

发表于由

上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Maleimide PEG aldehyde

Cat. No. PG2-ALML-3k
Specification 3400 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • White/off-white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
  • Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

Maleimide PEG aldehyde Cat. No. PG2-ALML-2k 2000 Da 10 mg修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

Maleimide PEG aldehyde

Cat. No. PG2-ALML-2k
Specification 2000 Da
Unit Size 10 mg
Price $385.00

Qty Add to Cart

Description:

Maleimide PEG aldehyde is one of Nanocs premier thiol (-SH) and N-terminal amine group pegylation reagents. Maleimide group reacts with sulfhydryl (thiol) group at pH 6.5-7.5 rapidly and specifically while aldehyde group reacts with the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. Pegylated maleimide and aldehydes are water soluble and all reactions can be carried out in aqueous buffer. Aldehyde reacts with n-terminus amine groups at a pH of from 5.0 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups.

Physical Properties:

  • White/off-white solid;
  • Soluble in warm water, DMSO, DMF;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 5- to 10-fold molar excess of Maleimide PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine and thiol free aqueous buffer, pH 5.0~6.5.
  • Maleimide PEG aldehyde stock solution: 20 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add maleimide PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

醛基功能载玻片(Aldehyde Functional Glass Slides)的用途

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醛基功能载玻片

醛基功能化载玻片是金畔生物功能玻璃产品之一。我们提供各种表面功能化玻片和盖玻片，可用于将生物分子附着在其上。官能团和涂层包括胺、羧酸、醛、环氧化物、生物素、链霉亲和素、NTA、马来酰亚胺、二硫化物、叠氮化物、炔烃以及聚D-赖氨酸和聚L-赖氨酸。金畔生物玻片和盖玻片采用独特的涂层技术制造，以确保其高反应性、均匀性和低非特异性吸收。这些玻片和盖玻片可用于微阵列制作、细胞培养生长和各种共焦显微镜工作。

规格：

表面功能性：Aldehyde (-CHO)

玻璃尺寸：25x75x1.1mm；

Aldehyde Functional Glass Slides

英文描述：

Aldehyde functionalized glass slides are one of jinphaibio premier functional glass products. jinphaibio provides various surface functionalized glass slides haid coverslips that chai be used to attach biomolecules on top of them. Functional groups haid coatings include amine, carboxylic acid, aldehyde, epoxide, biotin, streptavidin, NTA, maleimide, disulfide, azide, alkyne, as well as poly-D-lysine haid poly-L-lysine. jinphaibio glass slides haid coverslips were fabricated with unique coating technologies to ensure their high reactivity, uniformity haid low non-specific absorption. These glass slides haid coverslips chai be used for microarray fabrication, cell culture growth haid various confocal microscope work.

jinphaibio aldehyde functional glass Specifications:

Surface functionality: Aldehyde (-CHO);

Glass size: 25x75x1.1 mm;

Aldehyde density: 50~100 umol CHO/cm2.

醛基密度：50~100 umol acid/cm2

醛基功能载玻片(Aldehyde Functional Glass Slides)的用途

生物芯片醛基化载玻片

醛基功能化修饰的载玻片;Aldehyde Functional Glass Slides

醛基载玻片

载玻片表面醛基化修饰（-CHO）

醛基功能化修饰的载玻片;Aldehyde Functional Glass Slides

醛基,氨基,羧基,环氧基,多聚赖氨酸,peg,肝素修饰PS、PMMA、基础载玻片

Polystyrene Functional slide

醛基功能化基础载玻片Aldehyde Functional Glass Slides

载玻片表面醛基化修饰,便于氨基修饰的核酸探针和蛋白质性质的抗原抗体以及其 它物质的化学结合。

醛基修饰PS、PMMA、基础载玻片

醛基修饰PS载玻片

生物芯片表面修饰醛基

醛基(Aldehyde)修饰PS生物芯片，基质PS

醛基修饰PMMA载玻片   

生物芯片表面修饰醛基,基质PMMA

Methoxy PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-5k-1 5000 Da 1 g修饰性聚乙二醇

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Methoxy PEG Aldehyde, mPEG-CH2CHO

Cat. No. PG1-AL-5k-1 Methoxy PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-5k-1 5000 Da 1 g
Specification 5000 Da
Unit Size 1 g
Price $385.00

Qty Add to Cart

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
  • PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-5k-2 5000 Da 5 g修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

PEG Aldehyde, mPEG-CH2CHO

Cat. No. PG1-AL-5k-2 PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-5k-2 5000 Da 5 g
Specification 5000 Da
Unit Size 5 g
Price $785.00

Qty Add to Cart

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
  • PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-2k 2000 Da 500 mg修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

PEG Aldehyde, mPEG-CH2CHO

Cat. No. PG1-AL-2k PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-2k 2000 Da 500 mg
Specification 2000 Da
Unit Size 500 mg
Price $385.00

Qty Add to Cart

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
  • PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-1k 1000 Da 100 mg修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

PEG Aldehyde, mPEG-CH2CHO

Cat. No. PG1-AL-1k PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-1k 1000 Da 100 mg
Specification 1000 Da
Unit Size 100 mg
Price $385.00

Qty Add to Cart

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
  • PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-10k 10000 Da 1 g修饰性聚乙二醇

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上海金畔生物科技有限公司提供各种分子量和基团修饰性聚乙二醇定制服务。

PEG Aldehyde, mPEG-CH2CHO

Cat. No. PG1-AL-10k PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-10k 10000 Da 1 g
Specification 10000 Da
Unit Size 1 g
Price $385.00

Qty Add to Cart

PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
  • PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet

PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-20k 20000 Da 1 g修饰性聚乙二醇

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PEG Aldehyde, mPEG-CH2CHO

Cat. No. PG1-AL-20k PEG Aldehyde, mPEG-CH2CHO Cat. No. PG1-AL-20k 20000 Da 1 g
Specification 20000 Da
Unit Size 1 g
Price $385.00

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PEG Aldehyde Description:

Aldeyde PEG is an amine reactive PEG derivative that can be used to modify biomolecules via available amine groups. The reaction between aldehyde group with ε-amine of lysine residues and the α-amine at the N-terminus produces an intermediate Schiff base. Further reduction with hydride will form a stable C-N bond. PEG aldehydes react with amine groups at a pH of from 5.5 to 9.5. Higher pH will result in multiple Pegylation with both terminal and lysine groups. Aldehyde PEG is a commonly used PEG reagent for n-terminus pegylation in proteins or peptides.

Physical Properties:

  • Off-white/white solid or viscous liquid depends on molecule weight;
  • Soluble in regular aqeous solution as well as most organic solvents;

Storage Conditions:

  • Store at -20 0C

Reaction Procedures:

Generally, a 10- to 50-fold molar excess of PEG aldehyde over the amount of amine-containing material results in sufficient conjugation.

Materials Required:

  • Pegylation buffer: Amine-free aqueous buffer, pH 6~8.5.
  • PEG aldehyde stock solution: 100 mg in 1 mL conjugation buffer.
  • Washing solution: Distilled water or any aqueous buffer.

Reaction Steps:

Dissolve targeted materials in pegylation buffer. Estimate the concentration of primary amine groups on the targeted materials. Add PEG aldehyde stock solution to the targeted conjugation materials with the final concentration keep at least 10 mg/mL. Allow mixture agitates at room temperature for 2~4 hrs at room temperature or overnight 4 0C. After reaction, C=N bond can be reduced by sodium borohydride. Conjugate can be purified either by size exclusion chromatography or dialysis.

Documents
  • SDS
  • DataSheet