上海金畔生物科技有限公司提供预筛选人脐静脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
预筛选人脐静脉内皮细胞

product name：Human Umbilical Vein Endothelial Cells, Pre-screened(预筛选人脐静脉内皮细胞)；

cat no：ABC-TC3850；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description： 

AcceGen endothelial cells from large vessels are available from different locations, e.g. umbilical vein, umbilical artery, aorta, coronary artery, pulmonary artery, and saphenous vein.

In addition, AcceGen offers HUVEC isolated in standard Endothelial Cell Growth Medium and also in Endothelial Cell Growth Medium 2. The cells are supplied either from single donors or from pooled donors (from up to four different umbilical cords). Furthermore, HUVEC are available which are prescreened for VEGF (vascular endothelial cell growth factor) response.

Shortly after isolation, AcceGen Human Endothelial Cells from large vessels are cryopreserved at passage 1 or passage 2 (see page 5) using AcceGen’s proprietary, serum-free freezing medium, Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.

Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at AcceGen.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉内皮细胞，10个供体池 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉内皮细胞，10个供体池

product name：Human Umbilical Vein Endothelial Cells, 10-Donor Pool(人脐静脉内皮细胞，10个供体池)；

cat no：ABC-TC3852；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：HUVEC10-Donor Pools(人脐静脉内皮细胞，10个供体池), when grown in LIVas Medium, provide an ideal low-serum culture model, with or without human VEGF, for the study of angiogenesis, atherosclerosis or vascular biology without the concern of atypical results from a single donor. HUVEC 10-Donor Pools are cryopreserved as secondary cells to ensure the highest viability and plating efficiency.

Cell Features:

HUVEC are isolated from human umbilical cords, cultured on plastic and cryopreserved as primary cells.

HAoEC and HUVEC 10-Donor Pool are isolated from human aorta or umbilical cord, cultured on plastic and cryopreserved as secondary cells.

HPAEC are isolated from human pulmonary artery, cultured on plastic and cryopreserved as secondary cells.

HCAEC are isolated from human coronary arteries, cultured on plastic and cryopreserved as tertiary cells.

Human endothelial cells can be grown in low serum (2%) medium without phenol red or antimicrobials when cultured in VascuLife medium.

Culture human endothelial cells with or without VEGF.

human endothelial cells are extensively tested to meet quality standards and exhibit optimal performance.

AcceGen guarantees performance and quality.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉内皮细胞，新生儿 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉内皮细胞，新生儿

product name：Human Umbilical Vein Endothelial Cells, Neonatal(人脐静脉内皮细胞，新生儿)；

cat no：ABC-TC3853；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Human Umbilical Vein Endothelial Cells (HUVEC) are isolated from normal human umbilical vein. They are cryopreserved at the end of primary culture and can be cultured and propagated at least 16 population doublings.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉内皮细胞，联合供体 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉内皮细胞，联合供体

product name：Human Umbilical Vein Endothelial Cells, Pooled Donor(人脐静脉内皮细胞，联合供体)；

cat no：ABC-TC3854；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description： 

HUVEC-Umbilical Vein Endothelial Cells are available as single donor or pooled varieties.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉内皮细胞，刺激性补充脐血 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉内皮细胞，刺激性补充脐血

product name：Human Umbilical Vein Endothelial Cells, Stimulatory Supplement Cord Blood(人脐静脉内皮细胞，刺激性补充脐血)；

cat no：ABC-TC3856；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description： 

Human umbilical cord veins are obtained from healthy donors. Each cord vein is individually processed (or pooled for mixed products) to isolate endothelial cells through collagenase digestion and culture. Frozen HUVEC products are cryopreserved at the end of the primary culture. HUVEC are guaranteed to reach 15 population doublings when cultured with AcceGen’s recommended medium and supplement.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉平滑肌细胞 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉平滑肌细胞

product name：Human Umbilical Vein Smooth Muscle Cells(人脐静脉平滑肌细胞)；

cat no：ABC-TC3857；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Vascular smooth muscle cell is the cellular substate of most significant arterial disease. The increased growth potential of vascular smooth muscle cells represents one of the crucial anomalies responsible for the development of essential vascular diseases. New studies demonstrate that vascular smooth muscle cells express ICAM-1 and VCAM-1, which may contribute to the inflammatory reaction in the vascular wall and may actively be involved in the progression and stability of vascular disease. In vitro culture of human VSMC as a model of vascular research played a critical role and continues providing information in the pharmacology and the therapy of vascular diseases.HUVSMC from AcceGen Research Laboratories are isolated from human umbilical veins. HUVSMC are cryopreserved at the end of secondary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HUVSMC are characterized by immunofluorescent method with antibodies to ?-smooth muscle actin and desmin. HUVSMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUVSMC are guaranteed to further expand for 15 population doublings at the condition provided by AcceGen Research Laboratories.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉平滑肌细胞，新生儿 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉平滑肌细胞，新生儿

product name：Human Umbilical Vein Smooth Muscle Cells, Neonatal(人脐静脉平滑肌细胞，新生儿)；

cat no：ABC-TC3858；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Human umbilical vein smooth muscle cells (HUVSMC) are derived from the umbilical vein. They are cryopreserved at second passage. HUVSMC can be cultured and propagated 6 population doublings.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人静脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
人静脉内皮细胞

product name：Human Venous Endothelial Cells(人静脉内皮细胞)；

cat no：ABC-TC3868；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Primary Human Venous Endothelial Cells(人静脉内皮细胞)are pooled primary isolates from 250 individual human donor umbilical veins.

These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

These cells are available in both cryopreserved vials as well as in 25cm2 and 75cm2 proliferating culture flasks.

Each vial or flask of cells is shipped to Customer with Bac-O (antibiotic) and AcceSup™ (animal derived growth factors) or AcceSup-R™ (human recombinant growth factors) at no additional cost.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供猪静脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
猪静脉内皮细胞

product name：Porcine Vein Endothelial Cells(猪静脉内皮细胞)；

cat no：ABC-TC4038；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Porcine Vein Endothelial Cells(猪静脉内皮细胞)are derived from healthly porcinine vein tissue. Porcine Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen.

Porcine Vein Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供大鼠静脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
大鼠静脉内皮细胞

product name：Rat Vein Endothelial Cells（大鼠静脉内皮细胞）；

cat no：ABC-TC4239；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Rat Vein Endothelial Cells（大鼠静脉内皮细胞）from AcceGen are isolated from tissue of adult Sprague-Dawley Rats. Rat Vein Endothelial Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen.

Rat Vein Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供大鼠静脉成纤维细胞 ，欢迎访问官网了解更多产品信息和订购。
大鼠静脉成纤维细胞

product name：Rat Vein Fibroblasts（大鼠静脉成纤维细胞）；

cat no：ABC-TC4240；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Rat Primary Vein Fibroblasts from AcceGen are isolated from tissue of adult Sprague-Dawley Rats. Rat Primary Vein Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Vein Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.

Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供大鼠静脉平滑肌细胞 ，欢迎访问官网了解更多产品信息和订购。
大鼠静脉平滑肌细胞

product name：Rat Vein Smooth Muscle Cells（大鼠静脉平滑肌细胞）；

cat no：ABC-TC4241；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Rat Vein Smooth Muscle Cells（大鼠静脉平滑肌细胞）from AcceGen are isolated from tissue of Sprague-Dawley Rats. Rat Vein Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.

Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen.

Repeated freezing and thawing of cells is not recommended.

Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人脐静脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
人脐静脉内皮细胞

product name：Human Umbilical Vein Endothelial Cells（人脐静脉内皮细胞）；

cat no：ABC-TC3848；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Human Umbilical Vein Endothelial Cells (HUVECs,人脐静脉内皮细胞) are cells from the vein of umbilical cord. They have cobblestone morphology with large dark nuclei when observed under light microscope. HUVECs are the most commonly used source of endothelial cells due to their easy accessibility and affordability in price.

How to obtain human umbilical vein endothelial cells

HUVEC cells can be obtained from discorded umbilical cord after childbirth by enzymatic digestion of the lumen. They express many important endothelial signal molecules, for example von Willebrand factor (vWF), which is associated with vascular homeostasis regulation, blood platelets mediation and blood vessel wall tone.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.