上海金畔生物科技有限公司提供人肺动脉外膜成纤维细胞 ，欢迎访问官网了解更多产品信息和订购。
人肺动脉外膜成纤维细胞

产品名称：人肺动脉外膜成纤维细胞(Human Pulmonary Artery Adventitia Fibroblasts)；

货号：ABC-TC3773；

规格：1 vial；

生物安全等级：1；

运输信息：干冰运输；

储存：液氮；

产品描述：人肺动脉外膜成纤维细胞是来源于胚胎中胚层的间充质细胞。它们已被广泛用于广泛的细胞和分子研究。这主要是因为它们是最容易在培养中生长的细胞类型之一，并且它们的耐用性使它们能够接受从基因转染到显微注射的各种操作。有充分的证据表明，身体不同部位的成纤维细胞本质上是不同的。肺动脉血管对各种损伤的反应具有特征性的重塑模式，包括血管壁中层结缔组织的增加。血管成纤维细胞的功能紊乱是其发病机制之一。它们的增殖反应有助于在缺氧诱导的肺动脉高压发展过程中观察到的外膜增厚。埃森哲研究实验室的HPAAF是从人类肺动脉中分离出来的。HPAAF在第1代冷冻保存并冷冻输送。每个小瓶在1毫升体积中含有5×10^5个以上的细胞。HPAAF的特征是纺锤体形态和纤连蛋白抗体的免疫荧光法。HPAAF对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌呈阴性。HPAAF保证在AcceGen Research Laboratories提供的条件下进一步扩大15倍

质量控制：所有细胞的支原体、细菌、酵母和真菌检测结果均为阴性；

应用：仅用于科研。

上海金畔生物科技有限公司提供猪肺动脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
猪肺动脉内皮细胞

product name：Porcine Pulmonary Artery Endothelial Cells(猪肺动脉内皮细胞)；

cat no：ABC-TC4035；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Porcine Pulmonary Artery Endothelial Cells(猪肺动脉内皮细胞)are derived from healthly porcinine pulmonary vascular tissue. Porcine Pulmonary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen.

Porcine Pulmonary Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供猪肺动脉平滑肌细胞 ，欢迎访问官网了解更多产品信息和订购。
猪肺动脉平滑肌细胞

product name：Porcine Pulmonary Artery Smooth Muscle Cells(猪肺动脉平滑肌细胞)；

cat no：ABC-TC4036；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Porcine large artery smooth muscle cells(猪肺动脉平滑肌细胞)are derived from tunica intima and tunica media of healthy, fibrous plaque-free porcine arteries. They are cryopreserved at first passage (PCASMC at second passage) and can be cultured and propagated at least 16 passages.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供人肺动脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
人肺动脉内皮细胞

product name：Human Pulmonary Artery Endothelial Cells(人肺动脉内皮细胞)；

cat no：ABC-TC4046；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Cardiac Endothelial Cells include cells isolated from the aorta (HAEC), coronary artery (HCAEC), pulmonary artery (HPAEC), iliac artery (HIAEC), and microvascular cardiac cells (HMVEC-C). Cells are isolated from normal donors.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供大鼠肺动脉内皮细胞 ，欢迎访问官网了解更多产品信息和订购。
大鼠肺动脉内皮细胞

product name：Rat Pulmonary Artery Endothelial Cells（大鼠肺动脉内皮细胞）；

cat no：ABC-TC4201；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description： 

Rat Pulmonary Artery Endothelial Cells（大鼠肺动脉内皮细胞）from AcceGen are isolated from tissue of adult Sprague-Dawley Rats. Rat Pulmonary Artery Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and are delivered frozen.

Rat Pulmonary Artery Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供大鼠肺动脉成纤维细胞 ，欢迎访问官网了解更多产品信息和订购。
大鼠肺动脉成纤维细胞

product name：Rat Pulmonary Artery Fibroblasts（大鼠肺动脉成纤维细胞）；

cat no：ABC-TC4202；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Rat Primary Pulmonary Artery Fibroblasts（大鼠肺动脉成纤维细胞）from AcceGen are isolated from tissue of neonatal Sprague-Dawley Rats. Rat Primary Pulmonary Artery Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Cells are negative for bacteria, yeast, fungi, and mycoplasma. Rat Primary Pulmonary Artery Fibroblasts are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining and can be expanded by 2-4 passages at a split ratio of 1:2 under the cell culture conditions specified by AcceGen. Repeated freezing and thawing of cells is not recommended.

Standard biochemical procedures performed with fibroblast cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.

上海金畔生物科技有限公司提供大鼠肺动脉平滑肌细胞 ，欢迎访问官网了解更多产品信息和订购。
大鼠肺动脉平滑肌细胞

product name：Rat Pulmonary Artery Smooth Muscle Cells（大鼠肺动脉平滑肌细胞）；

cat no：ABC-TC4203；

Size/Quantity：1 vial；

Biosafety Level：1；

Shipping Info：Dry Ice；

Storage：Liquid Nitrogen；

Description：Rat Pulmonary Artery Smooth Muscle Cells（大鼠肺动脉平滑肌细胞）from AcceGen are isolated from tissue of Sprague-Dawley Rats. Rat Pulmonary Artery Smooth Muscle Cells are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in AcceGen’s Cell Culture Medium for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1×10^6 cells per ml and is delivered frozen.

Cells are negative for bacteria, yeast, fungi, and mycoplasma and are characterized by immunofluorescent staining with antibodies to α-smooth muscle actin.

Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by AcceGen.

Repeated freezing and thawing of cells is not recommended.

Standard biochemical procedures performed with smooth muscle cell cultures include the assays of cell to cell interaction, PCR, Western blotting, immunoprecipitation, immunofluorescent staining, immunofluorescent flow cytometry or generating cell derivatives for desired research applications.；

Quality Control：All cells test negative for mycoplasma, bacteria, yeast, and fungi；

Application：For research use only.