人口腔上皮细胞

上海金畔生物科技有限公司提供人口腔上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人口腔上皮细胞

详情介绍

product name:Human Oral Epithelial Cells(人口腔上皮细胞);

cat no:ABC-TC4365;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:The oral epithelium is a mucous layer in the mouth consisting of stratified squamous epithelial cells either keratinized or nonkeratinized. The oral epithelium and an underlying layer of lamina propria together form the mucosal epithelium that act as a physical barrier to external environment. For instance, the oral gingival epithelium act as a main barrier against bacterial biofilm that triggers gingivitis and periodontitis. Human primary oral epithelial cells are mainly distributed in the buccal part of oral cavity and undergoes continual regeneration to compensate for the damage caused by daily chewing.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

                       

人结肠上皮细胞

上海金畔生物科技有限公司提供人结肠上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人结肠上皮细胞

详情介绍

product name:Human Colonic Epithelial Cells(人结肠上皮细胞);

cat no:ABC-TC3570;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Colonic epithelium人结肠上皮细胞 cover the colonic mucosal surface and maintain the gut homeostasis by harboring the commensal bacteria in the outer mucus layer. Most colonic epithelial cells are absorptive colonocytes distributed in the crypt top, composed of specilised lineages including secretory goblet and enteroendocrine cells (EECs). They not only coordinate gut absorptive and secretory process, but also control mucosal immunity by presenting antigens to T cells. Dysregulation of the colonic homeostasis is often associated with intestinal inflammation, which underpins various intestinal disorders including bacterial infection, inflammatory bowel disease (IBD) and colonic cancer.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

                        

人大肠上皮细胞

上海金畔生物科技有限公司提供人大肠上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人大肠上皮细胞

详情介绍

product name:Human Large Intestine Epithelial Cells(人大肠上皮细胞);

cat no:ABC-TC3667;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:This cell line is available in limited quantities. Please contact us for more information.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

人小肠上皮细胞

上海金畔生物科技有限公司提供人小肠上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人小肠上皮细胞

详情介绍

product name:Human Small Intestine Epithelial Cells(人小肠上皮细胞);

cat no:ABC-TC3814;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description: 

Lining on the intestinal lumen surface, human small intestinal epithelial cells (IECs,人小肠上皮细胞) play a crucial role in food digestion, nutrient absorption and more importantly, constitutes a physical barrier that separates host from commensal microbiota. The crypts and villi border the surface area of intestinal epithelium and homes a variety of specialized epithelial lineages including absorptive enterocytes, enteroendocrine cells, goblet cells and Paneth cells. Unique to small intestine, Paneth cells lines in the base of crypts and secretes numerous antimicrobial peptides (AMPs) that strictly regulate antimicrobial resistance. Deregulated intestinal barrier function is often associated with increased risk of intestinal inflammation, systematic immune activation, development of inflammatory bowel disease (IBD) and increased sensitivity to chronic viral infections.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

                        

人肠上皮细胞

上海金畔生物科技有限公司提供人肠上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人肠上皮细胞

详情介绍

product name:Human Intestinal Epithelial Cells(人肠上皮细胞);

cat no:ABC-TC3879;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Human Intestinal Epithelial Cell Features:

Human Intestinal Epithelial Cells (IEC,人肠上皮细胞) are a layer of cells that line the luminal surface of intestinal epithelium. Stem cells located in crypts continuously produce new IECs at the base of the intestinal glands so that IECs could renew and migrate every 4-5 days.


Human Intestinal Epithelial Cells Function:

Intestinal epithelial cells play an important part in the maintenance of the symbiotic relationship between gut microbiota and the host. IECs support the food digestion, nutrient absorption, protection of organism from bacteria and toxins and coordination of appropriate immune responses etc. IECs function as a barrier through constructing mucosal barriers, secreting immunological mediators like cytokines and chemokines, and delivering bacterial antigens.

The dysfunction of intestinal epithelial cells will cause diseases such as inflammatory bowel disease (IBD), celiac disease, and diarrhoeal infection.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

                        

人阴道上皮细胞

上海金畔生物科技有限公司提供人阴道上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人阴道上皮细胞

详情介绍

product name:Human Vaginal Epithelial Cells(人阴道上皮细胞);

cat no:ABC-H0005Y;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Human Vaginal Epithelial Cells (人阴道上皮细胞are the inner lining of the vagina consisting of multiple layers of (squamous) cells, may be used to study cellular physiology of the reproductive tract, cellular response to infectious agents, and other areas of research.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

人支气管/气管上皮细胞–COPD

上海金畔生物科技有限公司提供人支气管/气管上皮细胞–COPD ,欢迎访问官网了解更多产品信息和订购。
人支气管/气管上皮细胞–COPD

详情介绍

product name:Human Bronchial/Tracheal Epithelial Cells – COPD(人支气管/气管上皮细胞–COPD);

cat no:ABC-H0098X;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:COPD Airway Cells include lung fibroblasts, bronchial epithelial and bronchial smooth muscle cells. Bronchial epithelial cells from COPD donors are isolated from epithelial lining of airways above bifurcation of the lungs. Cryopreserved Diseased Human Bronchial Epithelial cells from COPD donors test negative for mycoplasma, bacteria, yeast, and fungi. HIV – 1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

人支气管/气管上皮细胞-哮喘

上海金畔生物科技有限公司提供人支气管/气管上皮细胞-哮喘 ,欢迎访问官网了解更多产品信息和订购。
人支气管/气管上皮细胞-哮喘

详情介绍

product name:Human Bronchial/Tracheal Epithelial Cells – Asthma(人支气管/气管上皮细胞-哮喘);

cat no:ABC-H0099X;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:

Asthmatic Airway Cells include lung fibroblasts, bronchial epithelial and bronchial smooth muscle cells. Bronchial epithelial cells are isolated from epithelial lining of airways above bifurcation of the lungs. Cryopreserved Diseased Bronchial Epithelial cells from asthma donors test negative for mycoplasma, bacteria, yeast, and fungi. HIV – 1, hepatitis B and hepatitis C are not detected for all donors and/or cell lots.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

人肾上皮细胞

上海金畔生物科技有限公司提供人肾上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人肾上皮细胞

详情介绍

人肾上皮细胞

product name:Human Renal Epithelial Cells;

cat no:ABC-TC3782;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Human Renal Epithelial Cells(Human kidney epithelial cells,人肾上皮细胞) are derived from whole kidneys that have been dissociated into single cells and purified in culture. Human kidney epithelial cells are from a single donor. These are highly purified epithelial cells as determined by specific epithelial cell markers. Human kidney epithelial cells enable researchers to study the development and biochemical function of the kidneys. In addition, they are an ideal cell for studying absorption and secretion that occurs in the kidneys.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

人膀胱上皮细胞

上海金畔生物科技有限公司提供人膀胱上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人膀胱上皮细胞

详情介绍

产品名称:人膀胱上皮细胞

product name:Human Bladder Epithelial Cells;

cat no:ABC-TC4367;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description: 

Human Primary Bladder Epithelial Cells(人膀胱上皮细胞) from AcceGen are isolated from normal human bladder tissue. Human Primary Bladder Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen’ Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells at passage 3 are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. Cells are incubated with EpCAM-1 (CD326) antibody, following the application of magnetic beads pre-coated with secondary antibody.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

上海金畔生物代理Accegen在中国的业务。

上海金畔生物科技有限公司:

一、辐射五大服务方向:

国内试剂耗材经销代理;

国外试剂订购(直订或境外代理);

基因组测序(二代、二代+三代);

基因分型(SNP检测):低/中/高通量项目;

生物样本运输;


二、多家代理品牌,涉及30多种,经营代理500多个品牌:

Accegen、Intact Genomic、Sigma、Epigentek、ThermoFisher、DNA Genotek、USA Scientific……

所有生物试剂耗材都可以进口,特别是冷门品牌,价格优,货期短,可加急。大部分客户我们提供货到付款服务,上海金畔自成立依靠过硬的服务和良好的信誉与各大企事业单位、科研单位建立长期合作。

专业订购国内外试剂耗材,常备现货,一手货源,欢迎合作

                        

人肺泡上皮细胞(HPAEpiC)

上海金畔生物科技有限公司提供人肺泡上皮细胞(HPAEpiC) ,欢迎访问官网了解更多产品信息和订购。
人肺泡上皮细胞(HPAEpiC)

详情介绍

人肺泡上皮细胞(HPAEpiC),也称为人肺细胞,是从人肺组织中分离出来的。人肺泡上皮细胞排列在肺泡中,并由称为I型肺泡细胞(AT1)和II型肺泡(AT2)的2种上皮细胞亚型组成。

1型与2型肺细胞


AT1细胞是大型鳞状细胞,占肺泡表面积的95%以上,II型细胞比I型细胞小得多,分散在I型和相邻的肺泡间隔连接处。I型肺细胞形成薄空气-血液屏障的上皮成分,而II型肺细胞分泌表面活性剂,形成脂肪膜以降低表面张力。AT2细胞也可以分化以替代受损的AT1细胞。II型肺泡细胞含有Na+、K+-ATP酶和阿米洛利敏感性上皮Na+通道。


为什么选择AcceGen的人肺细胞?


AcceGen提供冷冻保存的原代肺泡上皮细胞,以获得理想的生存能力和电镀效率。每个小瓶含有1ml体积的>0.5 x 10^6个细胞。AcceGen人肺泡上皮细胞通过免疫荧光法与抗体E-cadherin和ZO-1进行鉴定。细胞可在我们推荐的肺泡上皮培养基中进行未来培养。


纯化的人II型肺泡上皮细胞和永生化的人肺泡上皮上皮细胞系也可从AcceGen获得。

原代乳腺上皮细胞*培养基

上海金畔生物科技有限公司提供原代乳腺上皮细胞*培养基 ,欢迎访问官网了解更多产品信息和订购。
原代乳腺上皮细胞*培养基

详情介绍

产品名称原代乳腺上皮细胞*培养基Human Mammary Epithelial Cell Medium)
产品说明原代乳腺上皮细胞*培养基Human Mammary Epithelial Cell Medium)是一种促进人乳腺原代上皮细胞体外生长的培养基。该产品是一种无菌的液体混合系统,含有维持目的细胞生长所必需的氨基酸、维生素、有机和无机化合物以及生长因子等。培养基基于碳酸氢盐的缓冲体系,在5%CO2/95%空气的培养箱中平衡时,pH值为7.4。该培养基的配方可以提供一个合适的营养环境,选择性地促进体外人乳腺原代上皮细胞的生长和扩增。

产品优势
(1) 培养成功率高

(2) 体外持续扩增

(3) 保持肿瘤原始病理特征

(4) 药敏结果与临床数据接近

使用说明:
使用前准备
1)15mL无菌离心管、移液器、移液管、无菌枪头等表面消毒后放入超净工作台中紫外照射30min。
2)提前30min从4℃冰箱取出*培养基平衡至室温,提前从-20℃冰箱取出所需体积的细胞外基质胶冰上融化。
乳腺原代细胞培
1)采用预冷的DMEM/F12培养基将细胞外基质胶(Corning#356231)按1∶ 50比例稀释,至少提前30min 将稀释后的基质胶预铺在培养瓶/板底部表面,并使其*覆盖底部表面,30~60 min后吸干备用。
2)将分离并计数后的细胞悬液参考表1中细胞数量接种于培养瓶/板,补加*培养基至所需体积,轻轻摇晃混匀,表面消毒后放置于培养箱,37℃、5%CO2条件下进行培养。
3)原代细胞初次接种后2-3天内勿动(利于细胞贴壁)。

原代乳腺上皮细胞传代
1)镜下观察细胞形成克隆,且汇合度达80~90%时即可传代。
2)培养箱中取出细胞,弃去旧培养液,加入适量TrypLE*Express酶(Gibco#12605010)37℃孵育,10~20min后取出,轻拍培养瓶/板侧面,显微镜下观察细胞消化情况。
3)细胞消化至细胞变圆并开始脱落时用含10%胎牛血清的DMEM培养基终止消化,并将细胞转移至离心管中,室温,300g,离心5min。
4)弃上清,以1-2mL*培养基重悬细胞,活细胞计数,将细胞悬液按适合的细胞密度接种于预铺有基质胶的培养瓶/板底部,预铺方法同上。
5) 补加*培养基至所需体积,轻轻摇晃混匀,表面消毒后置于培养箱,37℃、5%CO2条件下继续培养。
注意事项:

  1. 本产品在使用前需平衡至室温。

  2. 使用产品时应注意无菌操作,避免污染。

  3. 本产品中不含血清,含有抗生素,如无特别需要,不用额外添加血清和其他抗生素,可直接使用。

  4. 为保持本产品的理想使用效果,不宜将其过夜放置于室温或较高的温度环境中。

  5. 请于保质期内使用本产品,超出保质期后,将无法达到理想的使用效果。

  6. 样本若为肿瘤样本,建议使用术中样本,且肿瘤细胞比例越高(至少>50%),培养成功率越高,不推荐用于少量样本(如穿刺或者循环肿瘤细胞)的培养。

  7. 本产品仅用于科研用途,不能用于体外诊断。

原代乳腺上皮细胞条件重编程培养基

上海金畔生物科技有限公司提供原代乳腺上皮细胞条件重编程培养基 ,欢迎访问官网了解更多产品信息和订购。
原代乳腺上皮细胞条件重编程培养基

详情介绍

产品名称原代乳腺上皮细胞条件重编程培养基(Human Mammary Epithelial Cell Conditional Reprogramming Medium)
产品说明原代乳腺上皮细胞条件重编程培养基Human Mammary Epithelial Cell Conditional Reprogramming Medium是一种为促进乳腺原代细胞体外生长而设计的培养基。它是一种无菌的液体混合系统,含有必需和非必需的氨基酸、维生素、有机和无机化合物、激素、生长因子、微量矿物质等。该培养基产品基于碳酸氢盐的缓冲体系,在5%CO2/95%空气的培养箱中平衡时,pH值为7.4。该培养基的配方可以提供一个理想平衡的营养环境,选择性地促进体外人乳腺原代细胞的生长实现体外培养周期短、成本可控、操作便捷的目的。

产品优势
(1) 培养成功率高

(2) 体外持续扩增

(3) 保持肿瘤原始病理特征

(4) 药敏结果宇临床数据接近

使用说明

使用前准备
(1) y射线照射过的NIH-3T3细胞(40Gy辐照)。
(2)15mL无菌离心管、移液器、移液管、无菌枪头等表面消毒后放入超净工作台中紫外照射30min。提前30min从4℃冰箱取出原代细胞培养基平衡至室温。

乳腺原代上皮细胞培养
(1)人乳腺组织经消化酶消化后,经100微米筛网过滤后计数,按照表1中的细胞数接种在合适的培养器皿中,加入y 射线辐照后的NIH-3T3细胞(添加滋养细胞数量如表1和表2所示)。
(2)原代细胞初次接种后2-3天勿动(利于细胞贴壁)),培养过程中若培养基颜色变黄但细胞未长满时可换半液,镜下观察细胞未长满但3T3细胞已不足时,适量补充y射线辐照后的3T3细胞(一般补加初始数目的一半))。

∶以上表格仅供参考。倍增时间>48小时则定义为增殖较慢的细胞∶对干增殖较快细胞.可以综合倍增时间、接种器皿的规格以及培养周期来估算接种的细胞数量。具体实验需按具体情况对待。
乳腺原代上皮传代
(1)镜下观察细胞形成克隆,且汇合度达到80~90%时即可传代。
(2)培养箱中取出细胞,弃旧培养液,0.25%胰酶洗涤30秒后吸尽,再加入适量TrypLE™ Express (Gibco#12605010)37℃孵育,10-20min后拿出轻拍培养瓶/板侧面,显微镜下观察细胞消化情况。
(3)待消化至细胞变圆并开始脱落时用含10%胎牛血清的DMEM培养基终止消化,并将细胞转移至离心管中, 300g, 5min离心。

(4)弃上清,以1-2mL本细胞培养基产品重悬细胞,活细胞计数,将细胞悬液按适合的细胞密度接种于培养瓶/ 板
(5)之后,加入丫射线辐照后的NIH-3T3细胞(不同规格培养瓶/板底面积、接种原代细胞数量、添加滋养细胞 数量如表1和表2所示),在培养板/孔中补加乳腺原代上皮细胞培养基至所需体积,轻轻揺晃混匀,表面消毒后置培养箱,37℃、5% CO2条件培养其他步骤同上。
注意事项:

1.本产品在使用前需平衡至室温。
2.本产品中含有胎牛血清和原代细胞专用抗生素,如无特别需要不用额外再添加血清和双抗,可直接使用。
3.为保持本产品的理想使用效果,不宜将其过夜放置于室温或较高的温度环境中。
4.请于保质期内使用本产品,超出保质期,必须放弃使用。
5.原代细胞初次接种时会贴壁较慢和贴壁不牢,若无必要,尽量减少取出观察的次数,建议2-3天一次。
6.传代消化时切记不要消化过度,对细胞造成损伤,影响细胞状态。
7.接种细胞时注意细胞密度,不可过稀或过密。
8.使用产品时应注意无菌操作,避免污染。
9.本产品仅用于科研用途,不能用于体外诊断。

人乳腺上皮细胞基础培养基

上海金畔生物科技有限公司提供人乳腺上皮细胞基础培养基 ,欢迎访问官网了解更多产品信息和订购。
人乳腺上皮细胞基础培养基

详情介绍

Gibco™ Human Mammary Epithelial Cell Basal Medium (formerly ''''Medium 171''人乳腺上皮细胞基础培养基)

This medium contains essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. It does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. It is HEPES and bicarbonate buffered and designed for use in an incubator with an atmosphere of 5% CO2/95% air.


To support plating and long-term proliferation of normal human mammary epithelial cells, Human Mammary Epithelial Cell Basal Medium must be supplemented with Mammary Epithelial Growth Supplement (MEGS) (Cat. No. S0155). Each 5-mL bottle of MEGS contains all of the growth factors, hormones, and tissue extracts necessary for the culture of normal human mammary epithelial cells and is the correct amount of supplement for a 500 mL bottle of Human Mammary Epithelial Cell Basal Medium.


When a 500 mL bottle of medium is supplemented with MEGS, the final concentrations of the MEGS components in the supplemented medium are: BPE, 0.4% v/v; recombinant human insulin-like growth factor-I, 0.01 μg/mL; hydrocortisone, 0.5 μg/mL; and recombinant human epidermal growth factor, 3 ng/mL.

人扁桃体上皮细胞

上海金畔生物科技有限公司提供人扁桃体上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人扁桃体上皮细胞

详情介绍

product name:Human Tonsil Epithelial Cells(人扁桃体上皮细胞);

cat no:ABC-TC3834;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Tonsil epithelium is a heterogeneous lymphoid organ containing two different types of actively differentiating epithelia, the lining stratified squamous epithelium and the reticulated crypt, or lymphoepithelium. The tonsil crypts represent a specialized compartment, important in the immunological functions of the tonsil because of the immediate proximity of the epithelial and lymphoid tissues. In Situ staining of tonsil epithelium suggests that expression of the simple epithelial keratins K8, K18 and K19 is located to the crypt epithelial cells. Cell culture of tonsil epithelium produces heterogeneous squamous epithelial culture in which differentiation appears to be ongoing and keratin expression patterns are consistent with those found in tonsil epithelial cells in situ. HTonEpiC from AcceGen Research Laboratories are isolated from human normal tonsil tissue. HTonEpiC are cryopreserved at passage one culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HTonEpiC are characterized by immunofluorescent method with cytokeratine antibodies. HTonEpiC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HTonEpiC are guaranteed to further culture at the conditions provided by AcceGen Research Laboratories.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人气管上皮细胞

上海金畔生物科技有限公司提供人气管上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人气管上皮细胞

详情介绍

product name:Human Tracheal Epithelial Cells(人气管上皮细胞);

cat no:ABC-TC3839;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:The epithelium is the interface between the body and the external environment and covers all exterior surfaces and interior lumina. Depending on the tissue of origin, the functions of epithelial cells are diverse and include absorption, secretion, protection, transcellular transport, and sensation.

AcceGen offers a range of Epithelia Cells produced at AcceGen’s cell culture facility. The cells are isolated from normal human adult tissues of the airways (nasal mucosa, trachea, bronchi,and distal respiratory tract), breast (mammary gland), kidney (whole kidney and renal cortex), and the placenta (amniotic membrane).

Shortly after isolation, all AcceGen Human Epithelial Cells are cryopreserved at passage 2 (P2) using AcceGen’s proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing.

Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at AcceGen.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人尿管上皮细胞

上海金畔生物科技有限公司提供人尿管上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人尿管上皮细胞

详情介绍

product name:Human Urothelial Cells(人尿管上皮细胞);

cat no:ABC-TC3861;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:The urothelial cells, the cells lining the surface of the urinary bladder, are comprised of a unique cell type with high plasticity and a variety of cell functions. They are the first line of bladder defense and serve as an interface between pathogens that is equipped with several defense mechanisms to prevent adherence of pathogens and maintain impermeability to urinary solutes. The Urothel Epithelial Cells express both estrogen receptor-alpha and beta, epidermal growth factor receptor and fibroblast growth factor receptor, these receptors play a major role on urothelial cell response to injury and infection. They also release a number of cytokines and other immune system mediators and this newly recognized immunoregulatory potential of the urothelial cell warrants further investigation through the application of two recent developments: 1) the ability to culture normal urothelial cells in the laboratory and 2) the understanding of how immunoregulatory communications are generated for the biological functions of urothelial cells through signal transduction pathways.HUC from AcceGen Research Laboratories are isolated from the human bladder. HUC are cryopreserved at passage one and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HUC are characterized by immunofluorescent method with antibodies to cytokeratin-18, -19 and vimentin. HUC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HUC are guaranteed to further expand for 15 population doublings in the condition provided by AcceGen Research Laboratories.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人肺叶支气管上皮细胞

上海金畔生物科技有限公司提供人肺叶支气管上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人肺叶支气管上皮细胞

详情介绍

product name:Human Lobar Bronchial Epithelial Cells(人肺叶支气管上皮细胞);

cat no:ABC-TC3881;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description: 

Special edition cells are isolated from the tissue types described. Cells are sterility and virus tested. All tissues used for cell isolation are obtained under informed consent. AcceGen complies with the U.S. Health Insurance Portability and Accountability Act (HIPAA). Characterization standards for special edition cells have not been established.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

人前列腺上皮细胞

上海金畔生物科技有限公司提供人前列腺上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
人前列腺上皮细胞

详情介绍

product name:Human Prostate Epithelial Cells(人前列腺上皮细胞);

cat no:ABC-TC3995;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:Human Prostate Epithelial Cells (HPrEC,人前列腺上皮细胞), when grown in LIPro Medium, provides an ideal serum-free culture model, without retinoic acid, for the study of prostate cells. Common uses for HPrEC are studies of hormonal regulation of the prostate, regulation and control of the secretory function of prostate cells and as a control for the study of prostate cancer. HPrEC are cryopreserved as secondary cells to ensure the highest viability and plating efficiency. Our HPrEC are quality tested in LIPro Medium to ensure optimal serum-free growth over a period of at least 15 population doublings at rates equal to or greater than serum-supplemented media.


Cell Features:

HPrEC are cryopreserved as secondary cells, e.g. cells are isolated from

human prostate tissue and expanded twice in culture vessels before being

harvested for cryopreservation.

HPrEC proliferate in a serum-free, retinoic acid-free medium without

phenol red or antimicrobials when cultured in ProstaLifeTM medium.

HPrEC are extensively tested for quality and optimal performance.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.

猪角膜上皮细胞

上海金畔生物科技有限公司提供猪角膜上皮细胞 ,欢迎访问官网了解更多产品信息和订购。
猪角膜上皮细胞

详情介绍

product name:Porcine Corneal Epithelial Cells(猪角膜上皮细胞);

cat no:ABC-TC4029;

Size/Quantity:1 vial;

Biosafety Level:1;

Shipping Info:Dry Ice;

Storage:Liquid Nitrogen;

Description:The cornea is a unique tissue for two reasons: the transparency function and the synthesis of most of its proteins due to the cornea being an avascular tissue.;

Quality Control:All cells test negative for mycoplasma, bacteria, yeast, and fungi;

Application:For research use only.