Fluorescein labeled polyethylene glycol is a fluorescent PEG derivative which has a maximum absorption at 495 nm and emission around 515~520 nm. FITC group can be easily traced from its yellow color and green fluorescence. Additional functionality allows these fluorescent PEG derivatives to be easily conjugated to other molecule or material surfaces.
Physical Properties:
Organge/yellow solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated Protect from light. Avoid frequent thaw and freeze.
IMPACT（Intein Mediated Purificaiton with an Affinity Chitin-binding Tag）试剂盒利用经改良过的蛋白剪接元件（内含肽）进行一步亲和纯化重组蛋白（图1）。该系统与其它蛋白融合表达系统的主要区别在于：无需蛋白酶作用，即可将重组蛋白与其亲和标签（Tag）分离。
IMPACT 试剂盒能够融合表达含有内含肽及几丁质结合结构域（CBD）的蛋白，融合标签可以融合到目标蛋白的 C 端（pTXB1）或 N 端（pTYB21）（图 2）。在硫醇试剂（如 DTT）的参与下，内含肽进行特异性的自我剪切，释放出目的蛋白。pTXB1 载体还可用于表达和纯化 C 端含硫脂键的蛋白，并用于内含肽介导的蛋白连接（IPL，图 3）。IPL 反应，即表达蛋白连接，可通过一种天然肽键将 N 端为半胱氨酸的多肽或蛋白和一段细菌表达出的 C 端硫脂键蛋白连接起来，此反应可用于蛋白标记或半合成。有关 IMPACT 系统和 IPL 的更多信息请联系我们。 www.neb-china.com，www.neb.com 查询。
pTYB21 的内含肽标签包含酿酒酵母（Sce）VMA1 内含肽和几丁质结合域融合在目的蛋白的 N 末端。
This is a 13mm Hydrophilic PTFE membrane filter with a 0.1µm pore size. The Omnipore membrane is hydrophilic PTFE compatible with virtually all solvents, acids & alkaline solutions.
Folic acid PEG Biotin, also called Folate PEG biotin, is one of Nanocs reactive folic acid PEG derivatives. Biotin binds to avidin or streptavidin with high affinity. Biotin/streptavidin system has been widely used in bioassay development and other biomedical research. Folic acid PEG biotin allows the binding of pegylated folic acid to avidin/streptavidin quickly and efficiently. Folic acid functionalized substrates have been used for targeted drug delivery, imaging, and bioassay development.
Item specifications:
Appearance (color): Yellow;
Appearance (form): Solid;
Reactive group: Biotin;
Solubility: Soluble in water, DMSO, DMF.
Storage Conditions:
Store at -20 0C. desiccate. Protect from light. Avoid frequent thaw and freeze.
These Ultrafiltration Membrane Discs enable purification of proteins with a membrane NMWL of 30 kDa.“密理博Ultracel PLAC 圆片型超滤膜，再生纤维素，1 kDa NMWL，44.5 mm“
详情介绍
重要规格表
化学
主要应用
过滤器直径 (?)
NMWL
Regenerated Cellulose (RC)
Protein Purification, Protein Desalting, Protein Concentration
44.5 mm
1kDa
描述
产品目录编号
PLAC04310
商名
Ultracel
描述
Ultracel PLAC 圆片型超滤膜，再生纤维素，1 kDa NMWL，44.5 mm
产品信息
Device Configuration
Ultrafiltration Discs
过滤器代码
PLAC
过滤器类型
超滤膜
NMWL
1 kDa
应用
应用
These Ultrafiltration Membrane Discs enable purification of proteins with a membrane NMWL of 3 kDa. Use Ultracel regenerated cellulose membranes to concentrate or desalt dilute solutions.
IMPACT（Intein Mediated Purificaiton with an Affinity Chitin-binding Tag）试剂盒利用经改良过的蛋白剪接元件（内含肽）进行一步亲和纯化重组蛋白（图1）。该系统与其它蛋白融合表达系统的主要区别在于：无需蛋白酶作用，即可将重组蛋白与其亲和标签（Tag）分离。
IMPACT 试剂盒能够融合表达含有内含肽及几丁质结合结构域（CBD）的蛋白，融合标签可以融合到目标蛋白的 C 端（pTXB1）或 N 端（pTYB21）（图 2）。在硫醇试剂（如 DTT）的参与下，内含肽进行特异性的自我剪切，释放出目的蛋白。pTXB1 载体还可用于表达和纯化 C 端含硫脂键的蛋白，并用于内含肽介导的蛋白连接（IPL，图 3）。IPL 反应，即表达蛋白连接，可通过一种天然肽键将 N 端为半胱氨酸的多肽或蛋白和一段细菌表达出的 C 端硫脂键蛋白连接起来，此反应可用于蛋白标记或半合成。有关 IMPACT 系统和 IPL 的更多信息请联系我们。 www.neb-china.com，www.neb.com 查询。
pTYB21 的内含肽标签包含酿酒酵母（Sce）VMA1 内含肽和几丁质结合域融合在目的蛋白的 N 末端。
Ortho-pyridyl disulfide (OPSS) functionalized polyethylene glycol (OPSS PEG) is a thiol (-SH) group reactive PEG derivative that can be used to modify biomolecules or other materials via their available thiol groups. Ortho-pyridyl disulfide reacts with thiol group to form a stable disulfide bond while a thiol pyridyl group is released.
Physical Properties:
Off-white/white solid or viscous liquid depends on molecule weight;
Soluble in regular aqeous solution as well as most organic solvents;
Storage Conditions:
Store at -20 0C, dessiccated. Avoid frequent thaw and freeze.
