Dionex DNAPac PA200&PA200 RS Column 生物色谱柱

DNAPac PA200 & PA200 RS Nucleic Acid Column

Ultrahigh-Resolution Separations of Oligonucleotides

The Thermo Scientific^? DNAPac^? PA200 and PA200 RS are strong anion-exchange columns developed to provide unsurpassed high-resolution analysis and purification of synthetic oligonucleotides. Both supports are designed to resolve full length from n–1, n+1, and other failure sequences not possible with other columns. Retention times and selectivity can be controlled by choice of salt, pH, and solvent. Therefore, the separation can be tailored to the requirements of many different oligonucleotide analysis challenges. 

• Achieve n, n-1 resolution for oligonucleotides.
• Resolve oligonuleotide linkage and stereoisomers.
• Assay phosphorothioate purity.
• Attain selectivity control with eluent pH, salt, and solvent.
• Resolve RNA with aberrant (2’–5’) links from normal SSRNA.

The DNAPac PA200 is packed with a pellicular anion-exchange resin composed of an 8 μm diameter nonporous polymeric substrate to which quaternary amine-functionalized Thermo Scientific^? Dionex^? MicroBeads^? are bound. The rapid mass transport characteristics of this resin result in high-resolution oligonucleotide separations. 

The DNAPac PA200 RS is smaller, with 4 μm diameter resin for bio-UHPLC separations. These rapid separation (RS) columns are designed for use with the Thermo Scientific^? Dionex^? UltiMate^? 3000 BioRS system, with an operational backpressure limit of 10,000 psi. Columns packed with smaller particles improve resolution and offer better performance.

The DNAPac PA200 and PA 200 RS can be operated under denaturing conditions, such as high temperature (up to 85 °C), high-pH eluents (up to pH 12.5), or by the inclusion of chaotropic agents (such as Urea).

These columns offer the highest-quality phase stability over a broad pH range, deliver exceptional resolution, and support high-throughput separations. DNAPac PA200 and PA 200 RS columns support resolution of normal-length oligonucleotides (8- to 30- mer), extended-length oligonucleotides (30- to 70-mer), linear double-stranded DNA, and supercoiled versus nicked/linear DNA.

Analytical separations on the DNAPac PA200, 8?m, 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Thermo Scientific DNAPac PA200 HPLC (8?m) and DNAPac PA200 RS (Rapid Separation) UHPLC (4?m) columns for high-resolution separations of ssDNA and RNA.

Dionex DNAPac PA200&PA200 RS Column 生物色谱柱

DNAPac PA200 & PA200 RS Nucleic Acid Column

Ultrahigh-Resolution Separations of Oligonucleotides

The Thermo Scientific^? DNAPac^? PA200 and PA200 RS are strong anion-exchange columns developed to provide unsurpassed high-resolution analysis and purification of synthetic oligonucleotides. Both supports are designed to resolve full length from n–1, n+1, and other failure sequences not possible with other columns. Retention times and selectivity can be controlled by choice of salt, pH, and solvent. Therefore, the separation can be tailored to the requirements of many different oligonucleotide analysis challenges. 

• Achieve n, n-1 resolution for oligonucleotides.
• Resolve oligonuleotide linkage and stereoisomers.
• Assay phosphorothioate purity.
• Attain selectivity control with eluent pH, salt, and solvent.
• Resolve RNA with aberrant (2’–5’) links from normal SSRNA.

The DNAPac PA200 is packed with a pellicular anion-exchange resin composed of an 8 μm diameter nonporous polymeric substrate to which quaternary amine-functionalized Thermo Scientific^? Dionex^? MicroBeads^? are bound. The rapid mass transport characteristics of this resin result in high-resolution oligonucleotide separations. 

The DNAPac PA200 RS is smaller, with 4 μm diameter resin for bio-UHPLC separations. These rapid separation (RS) columns are designed for use with the Thermo Scientific^? Dionex^? UltiMate^? 3000 BioRS system, with an operational backpressure limit of 10,000 psi. Columns packed with smaller particles improve resolution and offer better performance.

The DNAPac PA200 and PA 200 RS can be operated under denaturing conditions, such as high temperature (up to 85 °C), high-pH eluents (up to pH 12.5), or by the inclusion of chaotropic agents (such as Urea).

These columns offer the highest-quality phase stability over a broad pH range, deliver exceptional resolution, and support high-throughput separations. DNAPac PA200 and PA 200 RS columns support resolution of normal-length oligonucleotides (8- to 30- mer), extended-length oligonucleotides (30- to 70-mer), linear double-stranded DNA, and supercoiled versus nicked/linear DNA.

Analytical separations on the DNAPac PA200, 8μm, 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Thermo Scientific DNAPac PA200 HPLC (8μm) and DNAPac PA200 RS (Rapid Separation) UHPLC (4μm) columns for high-resolution separations of ssDNA and RNA.

100mL实验室特级瓶德国DURANDURAN

20L棕色茶色实验室高硼硅玻璃蓝盖试剂瓶德国DURANDURAN

提倡高标准—Premium Bottle

Premium Bottle 是在 GL 45 蓝色螺旋盖配 DURAN? 标准瓶的基础上进一步开发出来的。除了采用性能（如耐化学性、稳定性及安全性）久经考验的原材料，在安全使用方面 Premium Bottle 作了全面的考量，使之更便于在实验室中制作。本品比常规实验室瓶的应用范围更广。

DURAN? Premium Bottle 的特性：

■ 瓶子的顶部配有特殊的耐高温螺旋盖和倾倒环， 可适用于 – 1 9 6 ℃至 +260 ℃；并内衬聚四氟乙烯镀膜的橡胶密封垫。瓶盖和倾倒环无染色剂成分，使之在烤箱、烘箱及消毒柜工作中无掉色污染问题，紧贴瓶盖的密封垫可避免渗漏。

■ Premium Bottle 的刻度标志精确到+/-5%，并刻有附加分刻度。其附加的反向刻度可易于同时识别当前瓶内容量及已倒出容量。

■ Premium Bottle 可确保 160K 的耐热冲击性能；经测试符合 TüV Rheinland 的严格规定，并通过 GS 符号确认，ISO 718，DMF 19757，本品可适用于高温消毒处理。

■瓶身、瓶盖及倾倒环符合 USP / FDA 标准

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

20L棕色茶色实验室高硼硅玻璃蓝盖试剂瓶德国DURANDURAN

20L棕色茶色实验室高硼硅玻璃蓝盖试剂瓶德国DURANDURAN

DURAN? 实验室棕色玻璃瓶

    DURAN? 实验室棕色玻璃瓶可防紫外线保护瓶内物质，本瓶适用于盛放易感光物质，并可长期贮存各种物质。

■ 紫外线吸收可达 500 nm 光波长

■ 采用特殊着色技术确保其耐化学性（从瓶身外部表面着色，不改变瓶内成分

■ 盖子需另外选配

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

15L德国DURAN高硼硅玻璃棕色实验室蓝盖试剂瓶

15L德国DURAN高硼硅玻璃棕色实验室蓝盖试剂瓶

DURAN? 实验室棕色玻璃瓶

    DURAN? 实验室棕色玻璃瓶可防紫外线保护瓶内物质，本瓶适用于盛放易感光物质，并可长期贮存各种物质。

■ 紫外线吸收可达 500 nm 光波长

■ 采用特殊着色技术确保其耐化学性（从瓶身外部表面着色，不改变瓶内成分

■ 盖子需另外选配

Dionex DNAPac PA100 Nucleic Acid Column 生物色谱柱

DNAPac PA100 Nucleic Acid Column

For n, n-1 Resolution of Single-Stranded Oligonucleotides

The DNAPac^? PA100 is a high-resolution anion-exchange column that provides single-base resolution. It is stable under denaturing conditions, rugged, reliable, and can be readily scaled up. The DNAPac PA100 is a 13 μm pellicular, nonporous polymeric resin with bound quaternary amine-functionalized MicroBeads^?. The rapid mass-transport characteristics of this resin result in very high-resolution oligonucleotide separations.

• Capable of n, n-1 resolution for oligonucleotides
• Resolves oligonucleotides with secondary structures
• Compatible with solvent, high pH, and high temperatures
• Analyzes phosphorothioate-based clinical samples
• Provides easy scale-up

The MicroBead resin used in DNAPac columns provides high-resolution separations to ensure maximum peak purity, and makes linear scale-up of your separation easy. For more complex separations, the polymeric properties of the DNAPac PA100 allows great flexibility to select ideal conditions, including high pH, high temperature, or chaotropic agents.

Synthetic oligonucleotides can be screened for production yield and failure sequences using the DNAPac PA100. Unit-base resolution of synthetic oligonucleotides to 60 bases and beyond has been demonstrated. The DNAPac PA100 can even be operated under denaturing conditions. Either high temperature (up to 90 °C) or high-pH eluents (up to pH 12.5) can be used to eliminate hydrogen bonding, allowing resolution of problem sequences such as self-complementary sequences or poly-G stretches.

Separates RNA / Double-Stranded DNA

The DNAPac PA100 is ideally suited for the purification and analysis of synthetic RNA. Failure sequences are easily separated from the full-length product. Double-stranded DNA, such as plasmids, or restriction fragments are also separated.

Analytical separations on the 4 mm diameter column can be scaled directly to larger diameter columns so preparative methods can be conveniently developed using small samples. The loading capacity of the DNAPac PA100 (4 × 250 mm) column is from 1 to 100 μg. Scaling the flow rate and sample size up for the 9 × 250 mm and 22 × 250 mm column yields essentially identical chromatography.

10L德国DURAN DURAN棕色实验室玻璃瓶DURAN蓝盖试剂瓶

10L德国DURAN DURAN棕色实验室玻璃瓶DURAN蓝盖试剂瓶

DURAN? 实验室棕色玻璃瓶

    DURAN? 实验室棕色玻璃瓶可防紫外线保护瓶内物质，本瓶适用于盛放易感光物质，并可长期贮存各种物质。

■ 紫外线吸收可达 500 nm 光波长

■ 采用特殊着色技术确保其耐化学性（从瓶身外部表面着色，不改变瓶内成分

■ 盖子需另外选配

Dionex CarboPac PA20 Carbohydrate Column 生物色谱柱

CarboPac PA20 Carbohydrate Column

Fast, Sensitive Mono- and Disaccharide Analysis

The Thermo Scientific Dionex CarboPac^? PA20 column line has been expanded to include a new 0.4 × 150 mm capillary column and a new Dionex CarboPac PA20 Fast Sialic Acid column.

Dionex CarboPac PA20 Carbohydrate Column 生物色谱柱

CarboPac PA20 Carbohydrate Column

Fast, Sensitive Mono- and Disaccharide Analysis

The Thermo Scientific Dionex CarboPac^? PA20 column line has been expanded to include a new 0.4 × 150 mm capillary column and a new Dionex CarboPac PA20 Fast Sialic Acid column.

Dionex CarboPac PA20 Carbohydrate Column 生物色谱柱

CarboPac PA20 Carbohydrate Column

Fast, Sensitive Mono- and Disaccharide Analysis

The Thermo Scientific Dionex CarboPac^? PA20 column line has been expanded to include a new 0.4 × 150 mm capillary column and a new Dionex CarboPac PA20 Fast Sialic Acid column.

Dionex CarboPac PA20 Carbohydrate Column 生物色谱柱

CarboPac PA20 Carbohydrate Column

Fast, Sensitive Mono- and Disaccharide Analysis

The Thermo Scientific Dionex CarboPac^? PA20 column line has been expanded to include a new 0.4 × 150 mm capillary column and a new Dionex CarboPac PA20 Fast Sialic Acid column.