mPEG-DBCO, PEG Dibenzylcyclooctyne

Methoxy PEG DBCO, MW 550, 750, 1000, 2000, 3400, 5000, 10k, 20k, 30k, 40k

Description:

mPEG DBCO, also called methoxy PEG DBCO, is a pegylated DBCO (dibenzocyclooctyne) derivative that can react with azide group without need any metal catalyst. The strain-promoted 1,3-dipolar cycloaddition of cyclooctynes and azides, also termed as the Cu-free Click reaction, is a bioorthogaonal reaction that enables the conjugation of two molecules in aqueous solution. PEG DBCO derivatives have excellent water solubility, possess fast kinetics and stability in aqueous buffer. They are one of the best pegylation reagents to modify proteins, peptides, antibodies, biopolymers and particles via copper free Click Chemistry reaction.

Product Specifications:

• Appearance: Light yellow to white solid, semi-solid depends on molecular weight of PEG;
• Solubility: Soluble in water, ethanol, DMSO, etc.;
• Reactivity: DBCO reacts with azide group (-N3).

Copper-free Click reaction procedures:

• Prepare the azide-containing reagents in reaction buffer.
• Add PEG DBCO reagents to azide containing reagents. Add 1 mol equivalent of limiting reagent to 1.5~3.0 mol equivalents of abundance reagent.
• Incubate the reaction at room temperature for 2~4 hours or 2~12 hours at 4 ⁰C.
• Purify conjugates either by dialysis or size-exclusion chromatograph.

Storage:

• Store at -20 ⁰C; Desiccate; Avoid frequent thaw and freeze.