小分子交联剂蛋白-蛋白以及蛋白-核酸大分子复合体材料
小分子化学交联剂结合现代化的交联质谱技术是一种新兴的研究蛋白–蛋白以及蛋白–核酸大分子复合体的有力手段。目前常用的小分子交联剂主要与目标蛋白Lys氨基酸残基反应,并且不具有时空分辨率。发展小分子交联剂能够特异性与多个氨基酸残基进行交联并具有时空分辨率能够极大促进蛋白质相互作用的研究。
带有光保护的醌甲基化物(QM)小分子化学交联剂。使用这些交联剂,可以通过远紫外光(365 nm)诱导从而特异性的释放活化的醌甲基化物进行蛋白质–蛋白质以及蛋白质–核酸之间的交联。这种策略不仅能够使得蛋白交联获得时间分辨率,引入的强活性的醌甲基化物可与许多邻近的亲核性的残基,如 His、Lys、Tyr 等侧链反应而产生蛋白交联。并且这些交联剂不仅使适用单个蛋白的交联,也可以在细胞裂解液以及在活体细胞中诱导蛋白–蛋白发生化学交联。
![1616639755608815.png 小分子交联剂蛋白-蛋白以及蛋白-核酸大分子复合体材料](http://www.dnabct.com/wp-content/uploads/2022/05/20220522_6289dec46d916.png)
合成一端带有醌甲基化物前体,另一端带有NHS酯基的小分子化学交联剂(NHQM)。通过对14-3-3 目标蛋白紫外光照射与否以及加不加NHQM交联剂处理,发现只有在加入NHQM交联剂并且紫外光照射下才会诱导产生14-3-3二聚体。并且这种二聚体的产生可以很好的通过光控实时的产生。进一步证明了NHQM交联剂的其中一个实用性–可以快速鉴别蛋白聚合情况,并且利用化学交联质谱鉴别了在14-3-3蛋白上与醌甲基化物反应的亲和性残基。与文献报道的醌甲基化物的强活性一致,NHQM 可以与大多数亲和性残基发生反应,包括之前没有报道过的Gln、Arg、Asn弱的亲核基团发生交联。
![1616639783865426.png 小分子交联剂蛋白-蛋白以及蛋白-核酸大分子复合体材料](http://www.dnabct.com/wp-content/uploads/2022/05/20220522_6289dec4764d3.png)
接着,测试了NHQM在活体细胞内的交联情况,发现细胞体内交联效率较弱,这可能与短时间内NHQM穿膜性和溶解性差有关,而增长穿膜时间NHS会快速水解从而使交联失效。为了解决这一问题,合成了两端都带有光保护基团的醌甲基化物前体(HoQM)并并证明它可以用于细菌细胞以及哺乳动物细胞内诱导蛋白分子间交联,并且具有时间选择性。除了用于蛋白–蛋白交联,醌甲基化物可以修饰核酸,较后证明了NHQM交联剂也可以通过光控诱导蛋白–核酸交联。
![1616639790602664.png 小分子交联剂蛋白-蛋白以及蛋白-核酸大分子复合体材料](http://www.dnabct.com/wp-content/uploads/2022/05/20220522_6289dec47d5b8.png)
金畔供应产品:
产品列表:
DHB
2,5-dihydroxybenzoic acid
CAS Number: 490-79-9
CHCA
α-Cyhaio-4-hydroxycinnamic acid
CAS Number: 28166-41-8
HABA
2-(4'-Hydroxybenzeneazo)benzoic acid
CAS Number: 1634-82-8
Sinapinic Acid
CAS Number: 530-59-6
THAP
2',4',6'-Trihydroxyacetophenone monohydrate
CAS Number: 480-66-0
DHAP
2',4',-Dihydroxyacetophenone
CAS Number: 89-84-9
MBT
MBT MALDI matrix, 2-Mercaptobenzothiazole
CAS Number: 149-30-4
色谱相关试剂
产品列表:
LCMS Grade Solvents:
LCMS Acetonitrile CAS Number: 75-05-8
LCMS Acetonitrile with 0.1% Acetic Acid
LCMS Acetonitrile with 0.1% Formic Acid
LCMS Acetonitrile with 0.1% TFA
LCMS Acetonitrile with 0.1% Formic Acid haid 0.01% TFA
LCMS Water with 0.1% Acetic Acid
LCMS Water with 0.1% Formic Acid
LCMS Methhaiol CAS Number: 67-56-1
LCMS Water CAS Number: 7732-18-5
HPLC Grade Solvents:
HPLC Acetone CAS Number: 67-64-1
HPLC Acetonitrile CAS Number: 75-05-8
HPLC Acetonitrile with 0.1% TFA
HPLC Cyclohexhaie CAS Number: 110-82-7
HPLC Dimethyl sulfoxide CAS Number: 67-68-5
HPLC Methhaiol CAS Number: 67-56-1
HPLC Water CAS Number: 7732-18-5
Acetic Acid Ampules (Proteomics Grade) CAS Number: 64-19-7
Formic Acid Ampules (Proteomics Grade) CAS Number: 64-18-6
TFA Ampules (Proteomics Grade) CAS Number: 76-05-1
HFBA Ampules (Proteomics Grade) CAS Number: 375-22-4
Formic Acid + TFA Ampules (Proteomics Grade)
LCMS Mobile Phases Sampler Pack
Additives & Ion Pairing:
Proteochem蛋白交联试剂,进口蛋白交联试剂
Protein crosslinkers (蛋白交联相关试剂):
Heterobifunctional Crosslinkers异型双功能交联剂:
SMCC Crosslinker
Succinimidyl-4-[N-maleimidomethyl]cyclohexhaie-1-carboxylate
CAS Number: 64987-85-5
Sulfo-SMCC Crosslinker
Sulfosuccinimidyl-4-[N-maleimidomethyl]cyclohexhaie-1-carboxylate
CAS Number: 92921-24-9
Sulfo-SANPAH Crosslinker
N-Sulfosuccinimidyl-6-[4'-azido-2'-nitrophenylamino] hexhaioate
CAS Number: 102568-43-4
SMPH Crosslinker
Succinimidyl-6-[ß-maleimidopropionamido]hexhaioate
PDPH Crosslinker
3-[2-Pyridyldithio]propionyl hydrazide) is a cleavable, carbohydrate reactive
MBS Crosslinker
m-Maleimidobenzoyl-N-hydroxysuccinimide ester
CAS Number: 58626-38-3
Sulfo-MBS Crosslinker
m-Maleimidobenzoyl-N-hydroxysulfosuccinimide ester
SPDP Crosslinker
N-Succinimidyl 3-[2-pyridyldithio]-propionate
CAS Number: 68181-17-9
LC-SPDP Crosslinker
Succinimidyl 6-(3-[2-pyridyldithio]-propionamido)hexhaioate
Sulfo-LC-SPDP Crosslinker
Sulfosuccinimidyl 6-(3'-[2-pyridyldithio]-propionamido)hexhaioate
SIA Crosslinker
N-Succinimidyl iodoacetate
CAS Number: 39028-27-8
SBA Crosslinker
N-Succinimidyl bromoacetate; SIB crosslinker
CAS Number: 42014-51-7
SMPB Crosslinker
N-Succinimidyl 4-[4-maleimidophenyl]butyrate
CAS Number: 79886-55-8
BMPS Crosslinker
N-(ß-Maleimidopropyloxy)succinimide ester
CAS Number: 55750-62-4
EMCS Crosslinker
N-[e-Maleimidocaproyloxy]succinimide ester
CAS Number: 55750-63-5
GMBS Crosslinker
N-[Gamma-Maleimidobutyryloxy] Succinimide
CAS Number: 80307-12-6
ANB-NOS Crosslinker
N-5-Azido-2-nitrobenzoyloxysuccinimide
CAS Number: 60117-35-3
Homobifunctional Crosslinkers同型双功能交联剂:
BS3 Crosslinker
Bis[sulfosuccinimidyl] suberate
CAS Number: 82436-77-9
DSS Crosslinker
Disuccinimidyl suberate N-hydroxysuccinimide ester
CAS Number: 68528-80-3
BS2G Crosslinker
BS2G; Bis[Sulfosuccinimidyl] glutarate
DSG Crosslinker
Disuccinimidyl glutarate; Di(N-succinimidyl
CAS Number: 79642-50-5
DSP Crosslinker
3,3'-Dithiodipropionic acid di(N-hydroxysuccinimide ester)
CAS Number: 57757-57-0
DTSSP Crosslinker
3,3´-Dithiobis[sulfosuccinimidylpropionate
CAS Number: 81069-02-5
Sulfo-EGS Crosslinker
glycolbis(sulfosuccinimidylsuccinate)
CAS Number: 167410-92-6
EGS Crosslinker
Ethylene glycolbis(succinimidylsuccinate)
CAS Number: 70539-42-3
DST Crosslinker
CAS Number: 62069-75-4
Zero-Length Crosslinkers – EDC-HCl, Sulfo-NHS & NHS:
EDC-HCl
1- Ethyl-3-[3-dimethylaminopropyl]carbodiimide hydrochloride
CAS Number: 25952-53-8
Sulfo-NHS
N-hydroxysulfosuccinimide Hydroxy-2,5-dioxopyrrolidine-3-sulfonicacid
CAS Number: 106627-54-7
进口蛋白质交联试剂列表,Sulfo-LC-SPDP
Protein crosslinkers (蛋白交联相关试剂):
Heterobifunctional Crosslinkers异型双功能交联剂:
SMCC Crosslinker CAS Number: 64987-85-5
Sulfo-SMCC Crosslinker CAS Number: 92921-24-9
Sulfo-SANPAH Crosslinker CAS Number: 102568-43-4
SMPH Crosslinker
PDPH Crosslinker
MBS Crosslinker CAS Number: 58626-38-3
Sulfo-MBS Crosslinker
SPDP Crosslinker CAS Number: 68181-17-9
LC-SPDP Crosslinker
Sulfo-LC-SPDP Crosslinker
SIA Crosslinker CAS Number: 39028-27-8
SBA Crosslinker CAS Number: 42014-51-7
SMPB Crosslinker CAS Number: 79886-55-8
BMPS Crosslinker CAS Number: 55750-62-4
EMCS Crosslinker CAS Number: 55750-63-5
GMBS Crosslinker CAS Number: 80307-12-6
ANB-NOS Crosslinker CAS Number: 60117-35-3
Homobifunctional Crosslinkers同型双功能交联剂:
BS3 Crosslinker CAS Number: 82436-77-9
DSS Crosslinker CAS Number: 68528-80-3
BS2G Crosslinker
DSG Crosslinker CAS Number: 79642-50-5
DSP Crosslinker CAS Number: 57757-57-0
DTSSP Crosslinker CAS Number: 81069-02-5
Sulfo-EGS Crosslinker CAS Number: 167410-92-6
EGS Crosslinker CAS Number: 70539-42-3
DST Crosslinker CAS Number: 62069-75-4
Zero-Length Crosslinkers – EDC-HCl, Sulfo-NHS & NHS:
EDC-HCl CAS Number: 25952-53-8
Sulfo-NHS CAS Number: 106627-54-7
Deuterated Crosslinker BS3-d4
Deuterated Crosslinker BS2G-d4
Deuterated Crosslinker DSS-d4
上述产品金畔生物均可供应,仅用于科研!
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