Dionex ProPac SCX and WCX Protein Column 生物色谱柱
简要描述：
戴安公司生产的仪器主要有离子色谱仪、高效液相色谱仪、快速溶剂萃取仪和氨基酸直接分析仪。其中离子色谱仪凭借着雄厚的技术优势和方法已广泛地应用于各个领域，其市场占有率达80%。与离子色谱相关的文章有90%均出自戴安公司生产的离子色谱仪。 戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取，该技术已在环境、药物、食品和聚合物工业等领域得到广泛应用。
| 型号 | 美国戴安SP5727 |
|---|---|
Strong and Weak Cation-Exchange Columns
for High-Resolution Separation of Proteins and MAbs
New! The Thermo Scientific ProPac? SCX column line has expanded to include the next generation ProPac SCX-20 column. This column provides unequalled high resolution of protein variants.
Please also see the Thermo Scientific MAbPac? SCX-10 column, a strong cation-exchange column designed specifically for high-resolution, high-efficiency analysis of monoclonal antibodies (MAbs) and associated variants. This column is complimentary to the industry-leading ProPac WCX-10 column for monoclonal antibody variant analysis. The MAbPac SCX-10 offers an alternative selectivity and provides higher resolution and efficiency for variant analysis of most monoclonal antibody samples than the ProPac WCX-10 column.
ProPac SCX-10 and WCX-10 strong and weak cation-exchange columns are based on a nonporous core particle that results in high-efficiency peaks and high-resolution separations of protein variants. A hydrophilic layer prevents unwanted secondary interactions. A grafted cation-exchange surface provides pH-based selectivity control and fast mass transfer for high-efficiency separation and moderate capacity.
- Characterization and quality control assessment of monoclonal antibodies and other proteins
- Unequalled resolution
- High-resolution separations with excellent sample recovery
- Unmatched column-to-column and lot-to-lot reproducibility
- Useful for characterization of related protein variants (e.g., deamidation and MAb lysine truncation variants)
- Highly-efficient peaks with excellent sample recovery
- Available in different dimensions and formats for flexibility in method development
The ProPac SCX-10 is a strong cation-exchange column with sulfonate functional groups, and the ProPac WCX-10 column has a carboxylate functional group. Both are based on 10 ?m nonporous, polymeric beads and coated with a proprietary hydrophilic layer.
All ProPac columns are manufactured and tested under the strictest specifications, resulting in unmatched column-to-column and lot-to-lot reproducibility. These columns are available as lot-select packages containing three columns from a single lot or three columns from three different lots, to assist quality control scientists in method validation.
| ProPac SCX-10 Strong Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications | |
|---|---|
| Maximum operating pressure | 3000 psi (21 MPa) |
| Maximum operating temperature | 60 °C Recommended operating temperature: <40 °C |
| Typical operating conditions | UV detection at 1 mL/min Recommended flow rate: 1 mL/min |
| phase compatibility | pH 2–12; 80% compatible with acetonitrile, acetone, methanol. Compatible with nonionic and zwitterionic detergents. Incompatible with cationic detergents. |
| Resin composition | 10-?m-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the sulfonate functional groups. |
| Anion-exchange capacity | < 100 ?g protein on-column, depending upon the protein (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| ProPac WCX-10 Weak Cation-Exchange Column for the High-Resolution Separation of Protein Isoforms Specifications | |
|---|---|
| Resin composition | 10-?m-diameter core bead (55% cross-linked) grafted with a pellicular layer to which are attached the carboxylate functional groups. |
| Anion-exchange capacity | < 100 ?g protein on-column, depending upon the protein (4 × 250 mm analytical column) |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Maximum operating pressure | 3000 psi (21 MPa) |
| Maximum operating temperature | 60 °C Recommended operating temperature: <40 °C |
| Typical operating conditions | UV detection at 1 mL/min Recommended flow rate: 1 mL/min |
| phase compatibility | pH 2–12; 80% compatible with acetonitrile and acetone. Compatible with nonionic and zwitterionic detergents. Incompatible with cationic detergents. |
| SCX Analytical Columns | |
|---|---|
| ProPac SCX-20 Analytical Column (4 x 250 mm) | 074628 |
| ProPac SCX-10 Analytical Column (4 x 250 mm) | 054995 |
| ProPac SCX-10 Analytical Column (2 x 250 mm) | 063456 |
| ProPac SCX-10 Semi-Preparative Column (9 x 250 mm) | 063700 |
| ProPac SCX-10 Semi-Preparative Column (22 x 250 mm) | SP5522 |
| ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (1 Lot of Resin) | SP5727 |
| ProPac SCX-10 Lot Select Column Set (4 x 250 mm) (3 Lots of Resin) | SP5728 |
| SCX Guard Columns | |
|---|---|
| ProPac SCX-20 Guard Column (4 x 50mm) | 074643 |
| ProPac SCX-10G Guard Column (4 x 50 mm) | 079930 |
| ProPac SCX-10G Guard Column (2 x 50 mm) | 063462 |
| WCX Analytical Columns | |
|---|---|
| ProPac WCX-10HT Analytical Column (4x 50mm) | 074600 |
| ProPac WCX-10 Analytical Column (4 x 100 mm) | SP5829 |
| ProPac WCX-10 Analytical Column (4 x 150 mm) | SP6703 |
| ProPac WCX-10 Analytical Column (4 x 250 mm) | 054993 |
| ProPac WCX-10 Lot Select 3 Column Set (4 x 250 mm) (1 Lot of Resin) | SP5512 |
| ProPac WCX-10 Lot Select 3 Column Set (4 x 250 mm) (3 Lots of Resin) | SP5513 |
| ProPac WCX-10 Analytical Column (2 x 250 mm) | 063472 |
| ProPac WCX-10 Semi-Preparative Column (9 x 250 mm) | 063474 |
| ProPac WCX-10 Semi-Preparative Column (22 x 250 mm) | SP5482 |
| WCX Guard Columns | |
|---|---|
| ProPac WCX-10G Guard Column (2 x 50 mm) | 063480 |
| ProPac WCX-10G Guard Column (4 x 50 mm) | 054994 |