甘油检测试剂盒 Glycerol Assay Kit
产品货号:K-GCROL
产品品名: 甘油检测试剂盒
英文品名 :Glycerol Assay Kit
规格型号: 70 assays per kit
甘油是食品加工业中通常使用的甜味剂和保湿剂,大多出现在运动食品和代乳品中。
The Glycerol test kit is a simple, reliable, rapid and accurate method for the measurement and analysis of Glycerol in beverages, foodstuffs and other materials.
Suitable for manual and microplate formats.
UV-method for the determination of Glycerol in foodstuffs,
beverages and other materials
原理: (glycerokinase)
(1) Glycerol + ATP → L-glycerol-3-phosphate + ADP
(pyruvate kinase)
(2) ADP + PEP → ATP + pyruvate
(L-lactate dehydrogenase)
(3) Pyruvate + NADH + H+ → L-lactic acid + NAD+
反应时间: 5min
检测限: 0.37mg/L
适用样品: 葡萄酒(葡萄汁)、啤酒、烈酒、醋、杏仁蛋白软糖、果汁、软饮料、牙膏、蜂蜜、烟、纸(硬纸板)、化妆品
优点:
l 新型的片剂形式增加了稳定性
l 价格低廉(每次检测成本)
l 所有试剂配制后的稳定性 >2 年
l 反应快
l 该方法已通过 OIV , MEBAK 以及德国和瑞士的认证
REFERENCES:
- Spinella, C. I. (1966). Modified enzymatic procedure for the routine determination of glycerol and triglycerides in plasma. J. Lipid Res ., 7,167-169 .
- Klopper, W. J., Angelino, S. A. G. F., Tuning, B. & Vermeire, H. A. (1986). Organic acids and glycerol in beer. J. Inst. Brew ., 92, 225- 228.
- Michal, G. (1976). Enzymatische analyse in der pharmazie. Acta Pharmaceutica Technologica , Suppl. 1, S, 151-162.
- Pfandl, A. & Menschig, D. (1984). Ein beitrag zur enzymatischen glycerin- und ethanol-bestimmung. Pharm. Ind ., 46, 403-407.
- Wieland, O. H. (1988). Glycerol. In Methods of Enzymatic Analysis (Bergmeyer, H. U., ed.), 3rd ed., Vol. VI, pp. 504-510, VCH Publishers (UK) Ltd., Cambridge, UK.