Dionex AminoPac PA1 Amino Acid Column 生物色谱柱
简要描述：
戴安公司生产的仪器主要有离子色谱仪、高效液相色谱仪、快速溶剂萃取仪和氨基酸直接分析仪。其中离子色谱仪凭借着雄厚的技术优势和方法已广泛地应用于各个领域，其市场占有率达80%。与离子色谱相关的文章有90%均出自戴安公司生产的离子色谱仪。 戴安公司的快速溶剂萃取仪15分钟之内仅用15毫升溶剂既可完成对10g样品的萃取，该技术已在环境、药物、食品和聚合物工业等领域得到广泛应用。
| 型号 | 美国戴安 P/N: 037023 |
|---|---|
AminoPac PA1 Amino Acid Column
For Specialized Amino Acid Applications with Postcolumn Derivatization
The AminoPac? PA1 column is a high-speed, pellicular, strong, anion-exchange column. The PA1 delivers high-efficiency separations as a result of its nonporous-core particle. Its strong anion-exchange characteristics are imparted by agglomerating surface MicroBead? particles that are functionalized with the quaternary ammonium functional group, resulting in a higher capacity than typically offered by nonporous resins.
- Resolves phosphorylated and other amino acids by anion-exchange chromatography
- Separates acid-labile amino acids under basic pH conditions
- Separates strongly acidic amino acids
- Requires OPA or ninhydrin postcolumn derivatization
The anion-exchange selectivity of the AminoPac PA1 can be used to resolve peaks that cannot be resolved using a cation-exchange column. For example, O-phosphorylated amino acids are resolved and eluted near the end of a hydrolysate chromatogram. Arogenic acid, a precursor to tyrosine and phenylalanine, decomposes under the acidic conditions required for cation-exchange separation or PITC derivatization, but it is stable in alkaline eluents used with AminoPac PA1 column.
The PA1 is particularly well suited for acidic and acid-labile amino acids and for amino acid pairs not compley resolved by cation-exchange chromatography.
| AminoPac PA1 Column for Specialized Amino Acid Applications with Postcolumn Derivatization Specifications | |
|---|---|
| Dimensions | AminoPac PA1 Analytical Column: 4 × 250 mm AminoPac PA1 Guard Column: 4 × 50 mm |
| Maximum operating pressure | 4000 psi (27.9 MPa) |
| Recommended operating temperature | Ambient |
| Typical operating conditions | 1.0 mL/min gradient Recommended flow rate: 1.0 mL/min |
| phase compatibility | Compatible with up to 5% common organic solvents. Recommended eluents: hydroxide, borate, and acetate. |
| Resin composition | 10-μm-diameter, pellicular substrate (2% cross-linked) agglomerated with a 180 nm MicroBead alkyl quaternary ammonium functionalized latex (20% cross-linked). |
| Anion-exchange capacity | N/A |
| Column construction | PEEK with 10-32 threaded ferrule-style end fittings. All components are nonmetallic. |
| Analytical Columns | |
|---|---|
| AminoPac PA1 Analytical Column (4 x 250 mm) | 037022 |
| Guard Columns | |
|---|---|
| AminoPac PA1 Guard Column (4 x 50 mm) | 037023 |